This is a summary list of all resource providers at University of Hawai'i Manoa . The list includes links to more detailed information, which may also be found using the eagle-i search app.
Telomeres are essential genetic elements that cap the ends of chromosomes. The replication of telomeres in proliferating cells is accomplished by a specialized ribonucleic acid enzymatic complex called telomerase. I have been studying telomere shortening and cell aging as well as telomerase biology, especially regulation of telomerase, for over a decade. More recently, as a post doctoral fellow in Dr. Irving Weissman’s lab at Stanford University, I became interested in stem cell biology, especially the regulation of telomerase in stem cells. I joined the Institute for Biogenesis Research (IBR) in the summer of 2003 as an assistant professor, where I am at present. My current research interests at the IBR are still in the area of stem cell biology and telomere/telomerase biology, and also include research on factors controlling and effecting long-term stem cell survival. I am particularly interested in figuring out how telomerase is regulated in stem cells, and what role telomerase dysregulation may have during perinatal development. I am also interested in the role that a new gene, Sirt1, may play in different types of stem cells. Sirt1 is an NAD-dependent protein deacetylase that effects both cell survival and has been implicated as a transcriptional regulator of telomerase as well. Stem cells which I am particularly interested in include blood stem cells, germ line stem cells and neuronal stem cells.
The Analytical Biochemistry resource was established to support and facilitate collaborative cancer research between the various disciplines at the Center by providing chemical analyses which contribute to understanding chronic diseases in our population. It also assists in the determination of the nature or structure of biological molecules as well as the quantification of those compounds. The facility is equipped with essential instruments needed to perform analytical chemistry, and modern, state-of-the-art technology for difficult-to-measure and ultra-low concentrated compounds.
The overall objective of the Animal Carcinogenesis Shared Resource is to provide the members of the Cancer Center with the ability to utilize animal carcinogenesis models in the implementation of their research projects. Animal in vivo experiments are required as a step of the processes of translational research by which the results of research done in the laboratory are used to develop new ways to diagnose and treat cancer.
Research: metagenomics simulation, metagenomics assembly, metagenomics annotation.
A 2000 square foot BSL-3 facility located at the University of Hawaii, JABSOM.
The Bachmann lab is located at the College of Pharmacy Department of Pharmaceutical Sciences at the University of Hawai‘i at Hilo. Research focuses primarily on a pediatric cancer called neuroblastoma. Molecular biology, cell biology and translational research is being conducted to elucidate new therapeutics and regimens for the treatment of relapsed patients with the goal to improve the currently low survival rates. Polyamine and proteasome inhibitors are investigated, and novel tumor promoting proteins (PRAF2) studied to better understand the underlying mechanisms which control tumor proliferation and metastasis. The Bachmann lab uses state-of-the-art new equipment to perform its research including the Perkin Elmer Operetta High Content Screening System with live cell capability as well as a 5 to 50 liter mammalian cell bioreactor to produce large protein quantities for downstream experimental analysis and protein crystallization. The Bachmann lab is an official member of the Neuroblastoma and Medulloblastoma Research Consortium (NMTRC) which conducts phase 1 and phase 2 clinical studies with neuroblastoma patients also using Dr. Bachmann's drug regimen. The lab also collaborates with several other U.S. and European laboratories to perform their research.
Dr. Bennett applies advanced technologies from genomics and bioinformatics to study dengue, hantavirus, influenza, and other viruses, and also bacteria such as leptospirosis and those found in mosquito vectors. She is especially interested in the nature of genetic mutations that give viruses the potential to cause epidemics or switch to new hosts.
Kapiolani Community College is part of the University of Hawaii System.
Research in the Berry lab is focused on selenoproteins, proteins containing the essential trace element, selenium, in the form of an unusual amino acid, selenocysteine. Most selenoproteins in higher organisms function either as antioxidants or in maintaining cellular redox balance. These functions explain the long-known antioxidant properties of selenium. In all selenoproteins whose functions are known, selenocysteine serves as the catalytic center of an enzyme, and its presence is required for optimal enzyme function.
"The Biological Electron Microscope Facility (BEMF) at the University of Hawai'i is a multi-user/service facility, administered by the Pacific Biosciences Research Center (PBRC). The mission of the BEMF is to provide biological-biomedical researchers with state-of-the-art instrumentation, training and services for high-resolution scanning electron microscopy, conventional and energy-filtering transmission electron microscopy, optical, fluorescence, and laser scanning confocal microscopy, and image analysis on a recharge basis. The facility receives partial support from the Pacific Biosciences Research Center and the UH Manoa Chancellor's Office."
Includes the Richard Allen Image Collection, a unique set of images taken with electron microscopes (http://www5.pbrc.hawaii.edu/allen/).
The University of Hawai’i John A. Burns School of Medicine Biostatistics & Data Management Core provides research design and biostatistical analysis collaborations and support to investigators from the John A. Burns School of Medicine, School of Nursing, as well as other schools and departments. The core serves as the biostatistics core/key function for several NIH institutional infrastructural grants at University of Hawaii, including RCMI Multidisciplinary And Translational Research Infrastructure eXpansion Program (RMATRIX), RCMI Bioscience Research Infrastructure Development for Grant Enhancement and Success Program (BRIDGES), RCMI Center for Native and Pacific Health Disparities Research (CNPHDR), and IDeA Networks of Biomedical Research Excellence (INBRE). Biostatistics services and collaborations include research design, data analysis, results dissemination, methodology development, and training and education. Biostatistical expertise covers epidemiologic investigations, bench science research, clinical studies and trials, and community-based investigations. Methodological research and the development of novel approaches are also conducted by this core. Consultations are provided to investigators and collaborative partnerships are formed to further develop biomedical research. This core also provides teaching and training in biostatistics and research design. Introductory and advanced biostatistics courses, research ethics courses, and seminars are offered to educate and foster growth in multidisciplinary and translational research. The biostatistics website also includes an online library of statistical tools and educational materials.
The Biostatistics Shared Resource (BSR) provides statistical consultation and support to the research enterprise of the University of Hawai‘i Cancer Center. The primary goal of the resource is to improve the quality of the research through sound statistical principles. The resource was established in 1995 and provides access to statistical support to all Center members on all aspects of research. The BSR provides short-term as well as long-term support for research projects. Support is provided to scientists in epidemiology, health behavior and quality of life research, and molecular and clinical oncology. The members of the Shared Resource often serve as co-investigators on epidemiology and cancer prevention and control projects. Consultation is available in the following areas: design, data collection, data analysis, statistical programming, interpretation and dissemination of results, and protocol review. The members of the resource have contributed to the publication of many peer-reviewed manuscripts from Center research projects. In addition, the statisticians work on methodological issues important to the research ongoing at UH Cancer Center. One area of interest is measurement error models that correct for bias in model parameter estimates when the exposure variable is measured with error. The role of dietary intake and sunlight exposure in cancer etiology are of interest at the Center, and these variables are measured with error. Another interest is in how susceptibility genes affect cancer risk, in combination with exposure data. Therefore, statistical techniques for the investigation of genes and lifestyle-gene interactions in cancer models are also studied.
Researchers in the Boisvert Lab are focused on exploring immunity and inflammation in the pathogenesis of atherosclerosis. Dr. Boisvert's current research focuses on:
1. IL-10 in Macrophage Foam Cell Formation
2. Macrophage Migration Properties
3. Role of ROCK in Atherosclerosis
4. Inflammasome in Macrophage Function
5. CD98 in Migration of Smooth Muscle Cells
6. MicroRNA regulation
Dr. Bursell has successfully developed instrumentation to noninvasively measure different aspects of ocular physiology including retinal blood flow, retinal vascular permeability and retinal leukostasis measurements. These technologies have also been used in clinical studies, and because these methodologies are common to both clinical and basic research, can facilitate the interpretation of clinical results. He has been a significant contributor, in collaboration with Dr. King and Dr. Aiello, in the translation of the protein kinase C inhibitor from bench top to clinical trial through research showing its significant impact on retinal physiology in both animal models and preliminary clinical studies. More recently, in collaboration with Dr. Feener, he has been investigating novel vitreal proteomes such as carbonic anhydrase, and characterizing their impact on retinal physiology and retinopathy development. Over the past 20 years he has conducted, or been a part of numerous clinical research studies. He is an internationally recognized authority in his field of research.
The COBRE Bioinformatics Facility at UHM offers access to high speed computer hardware and bioinformatics consultation. Research at the facility includes studying of evolutionary change in emergent dengue viruses associated with increasingly severe epidemics and confirm the impact of these changes in experimental models.
Offers services in the areas of qPCR, Microarray, Sequencing, and Other Genomic Services. May expand or remove services depending on need or interest. If you have recurring processes that you would like to automate or have us take over, we would be glad to sit down and talk with you to discuss how we can help you acheive your goals. We are aiming to be a self-sustaining entity consisting solely on a chargeback system. Our prices reflect the costs of equipment service contracts, potential and actual maintenence costs, as well as direct and indirect labor costs and administration. Our costs are kept low by being able to provide services in bulk with high throughput, it is therefore sometimes necessary to have minimum orders or fees in order to maintain a positive cost structure.
The of the goal of the Immunology Core of MCI, which functions as a fee-for-service facility, is to provide the Hawaii community with a service-oriented facility to meet the needs in the FACS research areas. We are equipped with BD FACSAria, BD FACSCalibur, GuavaEAsyCytePlus, Luminex 200, Beckman-Coulter ViCell, CTL-ImmunoSpot® S5 Core Analyzer, Bio-Rad and BioTek ELx808 ELISA readers and washers, Faxitron compact 43855D x-ray irradiation system, and Faxitron x-ray CP160 for in vivo animal work.
"The Center for Cardiovascular Research provides a Mouse Phenotyping Core for the use of investigators at the University, and by special arrangement, for investigators anywhere in Hawaii. We can provide murine echocardiography, blood pressure determinations, surgical procedures and phlebotomy, as well as assistance with mouse husbandry and genotyping."(http://www2.jabsom.hawaii.edu/mousecore/)
"The Transgenic and Embryonic Stem Cell Gene Targeting Core is a state-of-the-art facility with the expertise in the production of genetically altered subjects. Transgenic subjects carrying new or novel genes are created by microinjection of DNA into the pronuclei of fertilized eggs. Knock-out lacking specific genes of interest are created by homologous recombination in embryonic stem cells followed by injection into blastocysts to create chimeric subjects. Highly experienced personnel produce transgenic and knock-out subjects for UH investigators at very reasonable cost and with very short lead times." (http://www.ibr.hawaii.edu/tc.html)
There are two research projects in Dr. Callahan's lab. In the first a filamentous cyanobacterium is used to study the molecular genetics of cellular differentiation and the generation of periodic patterns. The second project looks at the contributions of bacteria, both commensals and pathogens, to the health of corals.
The Cancer Trials Support Unit (CTSU) is a project sponsored by the National Cancer Institute (NCI) for the support of a national network of physicians to participate in NCI-sponsored Phase III cancer treatment trials. The majority of these trials are sponsored by the adult Cooperative Clinical Trials Groups listed here.
Malaria is a major cause of global morbidity and mortality in tropical countries and most notably among infants, young children and pregnant women in sub-Saharan Africa. Our long term goal is to develop a safe and effective blood stage vaccine directed against merozoite surface protein-1 (MSP1), a major coat protein of P. falciparum merozoites. This goal requires development of two essential components of a malaria vaccine, (i) the production of an optimally immunogenic antigen capable of inducing a protective immune response, and (ii) the identification of an immunostimulatory and non-toxic adjuvant formulation that may be combined with this antigen to generate protective immunity to falciparum malaria. Previous studies in this laboratory have focused on developing a baculovirus recombinant 42 kDa MSP1 C-terminal polypeptide which is highly immunogenic and induces protection in a non-human primate model of P. falciparum malaria. One of our current research objectives is to improve the protective immune response to a P. falciparum MSP1-based malaria vaccine by combining sequences located within MSP1 blocks 4, 16, and 17 to induce protective immune responses to multiple MSP1 epitopes. Our second research objective is to evaluate the ability of a series of well-defined immunomodulators to stimulate an immune response to MSP1 with in vitro and in vivo biological activities against P. falciparum blood stages. We hypothesize that a multi-epitope MSP1 antigen formulated with specific combinations of TLR (Toll-like receptor) and NOD (nucleotide-binding oligomerization domain-like receptor) agonists will be effective in inducing a protective immune response to P. falciparum infection.
Church will investigate the linkages between microbial physiology and biogeochemical cycles, targeting the time and space dynamics of specific microbial groups that control transformations of carbon, nitrogen, and phosphorus. By merging rate measurements of organic matter production and consumption with molecular approaches to identify variability in gene expression, he seeks to elucidate how the activities, biomass, and diversity of functionally important groups of organisms contribute to biogeochemical variability in the oceans.
The Clinical Protocol and Data Management Shared Resource (CPDM) is a component of the Center’s Clinical Trials Unit that provides central management and oversight of all clinical trials activities of the Center.
The Shared Resource provides a range of management and quality control functions that are essential for conducting clinical trials research in Hawaii’s decentralized community practice setting. It includes a central location for all cancer protocols, a centralized database of protocol-specific data, an updated list of currently active protocols for the use of Center investigators, and status reports of protocols. It provides a centralized organization for activating and monitoring protocols throughout Hawaii’s multiple community offices and medical centers involving several Institutional Review Boards (IRBs). Quality control functions include: insuring subject safety and compliance with federal requirements through the Data and Safety Monitoring plan, insuring staff competency through training for research nurses, CRAs, clinical trials assistants and community office, clinic, and hospital staff engaged in Hawaii’s cancer clinical trials enterprise, and insuring recruitment of underserved minorities through focused outreach activities.
The vision of the College of Pharmacy at the University of Hawaii at Hilo is to drive improvement of healthcare in Hawaii and throughout the Pacific by focusing on Hawaii’s unique cultural, physical, and geographic features, by employing world-class faculty, and by graduating exceptional professionals.
The mission of the College is to educate pharmacy practitioners and leaders, to serve as a catalyst for innovations and discoveries in pharmaceutical science and practice for the promotion of health and wellbeing, and to deliver quality patient care.
The College’s mission is predicated on four over-arching goals:
To implement academic curricula that lead to a flagship Pharm.D. program, which produces graduates committed to serving people via science-based practice.
Accountability to the institute of medicine’s core competencies for the health professional workforce.
Conducting research that advances pharmaceutical sciences and makes a difference for humanity inclusive of effects on global health.
Cultivating culturally competent, intellectually inquisitive, self-directed, caring pharmacists, who are critical thinkers, problem solvers and life-long learners in a changing healthcare environment.
The values of the College are underlying ethics and accountability, community, diversity, scholarship, and life-long learning.
The College of Tropical Agriculture and Human Resources will actively help Hawai‘i diversify its economy, ensure a sustainable environment, and strengthen its communities, and will be the premier resource for tropical agricultural systems and natural resource management in the Asia-Pacific region.
The College of Tropical Agriculture and Human Resources is committed to the preparation of students and all citizens of Hawai‘i for life in the global community through research and educational programs supporting tropical agricultural systems that foster viable communities, a diversified economy, and a healthy environment.
Originally from New Zealand, Dr. Collier received her B.Sc. in Pharmacology from the University of Auckland (1998) and her PhD in Pharmacology from the same institution in 2003. Dr. Collier teaches Pharmacology to undergraduate and graduate students as well as Clinical Pharmacology for medical students and was awarded the University of Hawaii Regent’s Medal for Teaching in 2011. Within Pharmacology, her sub-specialty is drug metabolism and pharmacokinetics, primarily of the phase II (conjugation) enzymes, focused on pregnancy and pediatrics. A winner of the 2011 SimCYP award for Most Informative Report (“in recognition of scientific research that is leading the world in ADME, IVIVE, pharmacokinetics, modeling and simulation”), Dr. Collier uses a combination of wet laboratory work and in silico modeling to provide greater understanding of developmental pharmacology and improve drug/chemical safety. Dr. Collier’s research laboratory has been continuously funded by the National Institutes of Health, and private foundations since 2007. Along with her collaborators, she also performs research and publishes regularly in the fields of human and environmental toxicology and in endocrinology.
Our primary interest is in learning and the evolution of intelligence. We work mostly with honeybees, whose performance in a wide range of learning situations proves to be closely similar to that of vertebrates despite the remoteness of the evolutionary relationship and the vast differences in brain size and organization; broad functional convergence is indicated. In work with pigeons and fish, our primary concern is with the development of quantitative theories of learning that permit exact rather than merely ordinal predictions of experimental outcomes.
The Department of Human Nutrition, Food and Animal Sciences enhances nutrition and health by educating people and developing and disseminating science-based information to promote sustainable food production and animal systems appropriate for the Pacific Region.
The objectives of HNFAS are:
1. Ensure sustainable and profitable agricultural and food production systems.
2. Enhance research and instruction through the fundamental understanding of animal/human physiology.
3. Foster an integrated and interdisciplinary approach to learning that prepares students to be effective and productive citizens and life-long learners in a global society.
4. Have a safe and secure food system.
5. Have a healthy well-nourished population in the Pacific Region.
The mission of the Department of Native Hawaiian Health is to be an academic center of excellence committed to optimal health and wellness for all Native Hawaiian people through research, education, and quality health practices. To accomplish this mission, the department will actively seek partnerships with others in the community who share our mission and vision.
Dr. Donarchie investigates the diversity and role of microorganisms in the environment, focusing on both prokaryotes and eukaryotes in terrestrial and marine systems. Current projects explore potential relationships between bacteria (Archaea and Bacteria) and endemic Hawaiian marine invertebrates, Hawaiian marine Fungi and yeasts as sources of novel secondary metabolites, and biogeochemistry in Hawaiian lava tubes.
Dr. Ernst is a Professor at the JABSOM in the Dept. of Medicine, UH and an MR Physicist with extensive experience in the application of advanced MR techniques to clinical research in various brain disorders, especially in the areas of HIV and substance abuse.
The European Bioinformatics Institute (EBI) is an academic research institute located on the Wellcome Trust Genome Campus in Hinxton near Cambridge (UK), part of the European Molecular Biology Laboratory (EMBL).
The Evolutionary Genetics Core Facility (EGCF) at the Hawaii Institute of Marine Biology (HIMB) is located on Coconut Island in Kaneohe Bay, Oahu. The NSF EPSCoR-supported Core Facility provides technical services and support for the faculty and students doing research at HIMB and the University of Hawaii system, as well as other institutions worldwide. The EGCF provides access to various instrumentation and equipment and offers fee-based genomic services including sequence and fragment analysis. The EGCF manager is available to provide services and assist with questions regarding lab instrumentation or protocols.
Dr. Ruth Gates and her group focus their research on coral reefs, marine ecosystems that protect coastlines, support tourism and provide nutrition to many island nations. The global deterioration in the quality of these ecosystems has been widely reported over the last 40 years, reflecting the complex interaction between climate change stressors (thermal anomalies, ocean acidification and storms) and chronic or acute local impacts (coastal development, pollution and over-fishing). Although the future looks bleak, some corals survive, and even thrive in the same conditions that rapidly kill others. Our group seeks to better understand the biological underpinnings of this variability by defining traits that associate with environmental sensitivity and resistance in corals, and with the resilience (capacity to recover) of reefs. Our goal in conducting this research is to contribute basic and applied scientific knowledge that expands understanding of how coral reefs function, and informs the management and conservation of these beautiful but threatened ecosystems.
This lab also has an Experimental Manipulation Facility, which allows researchers to create climate change conditions (e.g. temperature, CO2, capacity for delta light & delta flow) in order to explore Marine living.
We use molecular approaches to conducted research addressing issues on the systematics of the native Hawaiian flora. Studies involve phylogenetic questions of species biogeography in the islands both in terms of from where they came from and how they are distributing among the islands. Studies utilize molecular markers to answer these questions, and the type of markers will depend upon the specific question being asked. Also of importance in studying plants, especially in Hawaii, is the consequences of small population sizes. Hawaii is the "Endangered Species Capital of the United State" with over 300 endangered plant species and many others that are "species of concern" by the US Fish & Wildlife Service. As such, the opportunities to examine rare plant population genetics from many different perspectives are incredible.
The purpose of the Genomics Shared Resource (GSR) laboratory is to provide expertise in the fast developing area of genomic analysis. In response to a growing need for genotyping services by a number of UH Cancer Center investigators, this laboratory was converted into a UH Cancer Center Shared Resource in 1999. Its current mission is to provide nucleic acid extraction, genotyping and expression profiling services in support of the peer-reviewed and IRB-approved research conducted by UH Cancer Center members.
Research in Dr. Gerschenson’s lab studies mitochondria in human cells and tissues. We are interested in adult and pediatric metabolic diseases (HIV and non-HIV insulin resistance, diabetes, obesity, fat, muscle, and liver tissue), neurological diseases (HIV peripheral neuropathy and HIV associated dementia), immunological diseases, and cancer. We conduct local, national, international clinical studies to obtain blood, cheek cells, fat tissue, urine, and cerebrospinal fluid, etc. to study mitochondrial genetics, energy metabolism, oxidative stress, cell death. We are also interested in how cytokines regulate mitochondrial genes via transcription and cell signaling.
HURL's facilities include two deep-diving (2000 m) submersibles Pisces V and Pisces IV, a remotely operated vehicle RCV-150, and the support ship R/V Ka'imikai-o-Kanaloa. The two submersibles are housed and maintained at Makai Pier.
HURL Data Archive: HURL provides a data package for each Pisces IV, Pisces V or RCV-150 dive. This package includes voice transcripts (Pisces IV & V only), graphs of environmental data (Pisces IV & V only), copies of videotapes with videologs, and copies of up to 250 slides with a photolog. The Data Archive is the custodian of the original data which remain the property of HURL. Databases of organisms and substrates observed on the dives are available for use by the principal investigator. Use of dive data by other scientists is subject to principal investigator approval for 2 years. HURL and NOAA-NURP reserve the right to use slides and video from dives for program promotion.
Dr. Ha's research focuses on biochemistry and minority education.
The Alcohol and Drug Abuse Division (ADAD) is the primary and often sole source of public funds for substance abuse treatment. ADAD's treatment efforts are designed to promote a statewide culturally appropriate, comprehensive system of services to meet the treatment and recovery needs of individuals and families. Treatment services have, as a requirement, priority admission for pregnant women and injection drug users.
Philosophy: Health, that optimal state of physical, mental, social and environmental well-being, is a right and responsibility of all of Hawaii's people.
* Promote health and well-being
* Prevent disease and injury
* Promote healthy lifestyles and workplaces
* Promote the strength and integrity of families and communities
To prevent pollution and promote and preserve a clean, healthy and natural environment
* Promote resource conservation (recycling)
* Protect and enhance air and water quality
To assure basic physical and mental health care (the five A's)
* Assured quality
* Ensure that core public health functions - assessment, policy, and assurance - are implemented or maintained.
* Ensure that federal mandates, including court-ordered settlements are satisfied.
* Ensure that resources are directed at those problems that pose the greatest risk to the public's health and the environment.
* Ensure that appropriate and cost-effective resources are dispersed geographically and satisfy principles 1 to 3.
* Ensure that the health department is the service provider of last resort for uninsurable populations and where there is no other satisfactory alternative.
The HMSA Foundation is committed to improving the health of Hawaii's people by supporting community and statewide programs.
HSFL was established in May 2007 within the School of Ocean and Earth Science and Technology (SOEST) and the College of Engineering (CoE) at the University of Hawaii. As a multidisciplinary research and education center HSFL brings together individuals from diverse areas to work on the exploration and understanding of the space environment. The mission of HSFL is to:
* Promote innovative engineering and science research for terrestrial and planetary space missions.
* Develop, launch, and operate small spacecraft from the Hawaiian Islands to accelerate the validation of new space technologies.
* Provide workforce training in all aspects of unmanned space missions.
* Build synergistic collaborations among educational, governmental, and corporate institutions interested in space exploration.
Hawaii is located in a unique position to become a low-cost gateway to space and to place the University of Hawaii as the only university in the world to have both satellite fabrication capabilities and unique, direct access to orbital space. This will enable diverse missions that study Earth's oceans and continents from low-Earth orbit, as well as the testing of engineering experiments in the hostile environment of space.
The Hawai'i Undersea Research Laboratory is the only U.S. deep submergence facility in the Pacific Rim tasked with supporting undersea research necessary to fulfill the mission, goals, and objectives of the National Oceanic and Atmospheric Administration (NOAA), along with other national interests of importance. Over 30 years of submersible operations have resulted in nearly 1900 dives representing 9300 hours underwater, and a benthic ecology database derived from in-house video record logging of over 125,000 entries based on 1100 unique deep-sea animal identifications in the Hawaiian Archipelago. With emerging interest in marine resources of the Pacific and renewable energy from the sea, HURL's contributions will continue to play an essential role in scientific research and advanced technology. HURL was established by Cooperative Agreement in 1980 between NOAA and the University of Hawai‘i, at which point it became a Regional Center in NOAA's Undersea Research Program (NURP). In 2009, President Obama signed Public Law 111-11 authorizing both NURP and Ocean Exploration (OE), and their administration under a merged program called the Office of Ocean Exploration and Research (OER). The Center supports highly-rated, peer-reviewed proposals to conduct undersea research in offshore and nearshore waters of the main and Northwestern Hawaiian Islands and waters of the central, southern, and western Pacific, including the new marine national monuments. In addition, HURL accepts funded requests from private, state, or federal agencies and participates in international collaborative research projects.
The Hawai‘i Institute of Marine Biology is a world-renowned marine research institute of the School of Ocean & Earth Science & Technology (SOEST) at the University of Hawai'i at Manoa. Situated on Moku o Lo'e (Coconut Island) in Kane‘ohe Bay, HIMB provides excellent opportunities for tropical marine research located just 15 miles from the main campus and downtown Honolulu.
HIMB offers cutting edge research facilities for faculty, students, and visiting scientists coupled with convenient access to a diverse range of marine environments. For example, Coconut Island is surrounded by 64 acres of coral reef designated by the state of Hawai‘i for research activities only as the Hawai‘i Marine Laboratory Refuge. Research at HIMB covers many disciplines of tropical marine science such as coral ecology, biogeochemistry, and evolutionary genetics. In addition, HIMB faculty are recognized authorities in marine diseases, neuroendocrinology, microbial organisms, and sensory systems of marine mammals and elasmobranchs. Visit our Faculty and Research pages to learn more about ongoing scientific activities at HIMB.
Graduate and undergraduate students conducting research on Coconut Island are most often enrolled in the departments of Zoology and Oceanography at the University of Hawai’i, but also include students from a variety of departments and programs across campus such as the departments of Microbiology, Geography, Molecular Biosciences and Bioengineering (MBBE), Human Nutrition, Food and Animal Sciences (HNFAS), and the Global Environmental Sciences program at the department of Oceanography.
The emphasis of Dr. Hemscheidt's research program is in the area of natural products chemistry, specifically biosynthesis and the isolation of natural products from plants and fungi.
Dr. Hernandez's research focuses on infection and cancer. Viruses associated with human cancers include human papillomavirus (HPV), hepatitis B and C, Epstein-Barr virus, human herpesvirus 8, and Kaposi's sarcoma. These infectious agents influence the development of cancer through varied mechanisms including interaction of viral genes with host tumor-suppressor genes, integration of virus into host DNA, and the establishment of persistent or latent infections. Understanding the role of host response to infection is also key to elucidating the mechanism of viral carcinogenesis.
The Hoang Lab currently has two areas of infectious disease research interests, focusing on the genetics and pathophysiology of i) Burkholderia pseudomallei and ii) Pseudomonas aeruginosa.
This a relatively new lab; funding NIH/NCCAM to research the mechanisms by which selenium influences T Helper Cells during Immune Responses. The major goals of this project are to characterize mechanisms by which Se affects CD4+ T cell activation and identify specific selenoproteins that mediate the effects of Se on CD4+ T cells.
Dr. Hui's research is based on the development of blood stage malaria vaccines; and studies on the use of different vaccine adjuvants for the malaria vaccines. Specifically, his focus is on the design of vaccines based on the Merozoite Surface Protein 1 (MSP1) by defining critical T and B epitopes of the molecule. His lab also evaluates the use of a variety of immunological adjuvants to enhance vaccine potency and at the same time define the critical pathway for adjuvants’ mode of action. The approaches also study the use of nanoparticle platforms for antigen delivery.
The goals of the Bioinformatics Core are:
* To increase the research capacity in bioinformatics in the State of Hawaii
* Provide hardware, access, and support to researchers who utilize genomic and proteomic data
* Provide a web-based gateway with access to a variety of bioinformatics applications, including available proprietary software such as Celera, and support for this software
* Undertake collaborative project with INBRE researchers to develop novel tools or applications that address specific needs
* Develop an academic program in bioinformatics with the John A. Burns School of Medicine (JABSOM)
* Recruit a full-time junior faculty member to lead the academic program
* Conduct training workshops and courses, and run a user support helpdesk
The Informatics Shared Resource (ISR) of the Cancer Research Center of Hawaii (CRCH) was established as a core facility in 2004. Its goal is to promote multidisciplinary collaboration, and enhance the research excellence and productivity of CRCH by providing access to biomedical informatics expertise and computational support to all members of the Center. The prime objective of the ISR is to facilitate the management, sharing, and integration of diverse data types in cancer research, as well as the synthesis and analysis of more focused data sets from the basic, clinical, genomics and population sciences. The bioinformatics group of the ISR supports a dedicated scientific computing network for computational cancer biology,and leads the development and application of advanced algorithms and software tools that enable Center investigators to take a more integrative approach to cancer research for the purpose of accelerating the translation of basic research results into the clinic.
The University of Hawai'i Insect Museum serves as a center for insect identification and systematics-based research. We have workspace, equipment, and resources for detailed taxonomic analysis, and researchers from many different laboratories are able to work and interact under its auspices.
The major focus of the IBR's research is reproductive and developmental biology, with an emphasis on assisted reproductive techniques (ART). IBR faculty use ART as a model to understand mammalian reproduction and development, and to develop new transgenic models for biomedical research. We also use animal models of ART to investigate and improve current ART usage in fertility clinics. Our research includes a wide range of studies from basic DNA structure to post-fertilization effects of ART on human embryonic development.
The IBR was founded by Dr. Ryuzo Yanagimachi, the first scientist to clone a mouse. He recruited Dr. Ward in 2000. Dr. Ward became the IBR Interim Director in 2004. Dr. Ward's laboratory focuses on the three dimensional structure of DNA in the cell, using sperm as a model system. Funding: NIH (COBRE). In 2008, Dr. Ward received a $10 million grant to develop the IBR into a world-renowned institute in reproductive biology.
Our laboratory utilizes fluorescence methodologies to elucidate dynamic aspects of biomolecules. We are currently studying dynamin, a large (98kDa) GTPase which functions to “pinch-off” membrane vesicles in pathways such as receptor mediated endocytosis and synaptic vesicle recycling. We carry out both in vitro and in vivo studies on the self-association modes of dynamin as well as its interaction with membranes and other proteins such as endophilin and Arc/Arg3.1. We have also been studying dynamins with mutations that cause motor disorders, specifically Centronuclear Myopathy and Charcot-Marie-Tooth disease. These studies, both in vitro and in living cells, are aimed at understanding the molecular basis for dynamin’s involvement in these disorders. Recently, we began a project to study, both in vitro and in vivo, the self-assembly of the protein Leucine Rich Repeat Kinase 2 or LRRK2. Mutations in the gene for LRRK2 are responsible for an autosomal dominant form of Parkinson’s Disease (PD). We also have a project on Botulinum Neurotoxin which involves biophysical studies on proteins forming the neurotoxin complex as well as development of in vitro and in vivo toxin assays based on Fluorescence Fluctuation Spectroscopy. Recently we have been developing the application of the phasor method, a visual approach to treatment of time-resolved fluorescence data, to in vitro systems such as intrinsic protein fluorescence.
- the S-Adenosylmethionine-Dependent Radical Enzymes
- the AdoMet Radical Enzyme Biotin Synthase
- in Vivo Iron-Sulfur Cluster Assembly
Dr. Kaholokula is interested in the study of biological, psychological, and socio-cultural factors (and their interplay) affecting the etiology and management of chronic diseases (and their risk factors) in Native Hawaiians and Pacific Islanders and in the designing and testing of behavioral interventions for primary and secondary prevention. His clinical interest is in behavioral assessment for clinical case formulation.
Dr. Kolonel's research focuses on the understanding the striking variations in cancer incidence and survival that are observed among the several different ethnic populations in Hawai‘i.
Current research projects focus on elastin, a vertebrate protein with remarkable biomechanical properties. The elastic properties of a number of physiological structures, such as blood vessels and skin, are believed to originate from elastin, an amorphous, crosslinked protein comprised largely of small hydrophobic amino acids. The three-dimensional structures of insoluble elastin remains elusive, as crystallographic tools and even the most sophisticated solution NMR spectroscopy cannot be used to study this insoluble protein. Therefore, for many years, the true nature of elasticity in biological systems has remained controversial, as none of the existing models could be confirmed or rebutted with high-resolution structural data.
Laboratory Support supplies support services for laboratory-based programs at the University of Hawai‘i Cancer Center. This resource is operated in order to streamline and make more centralized common laboratory needs.
The Laboratory for Microbial Oceanography is a research unit within the School of Ocean and Earth Science and Technology (SOEST) at the University of Hawaii at Manoa.
The Laboratory for Microbial Oceanography conducts basic research on microbial inhabitants of the sea, including bacteria, protozoans and unicellular algae. These studies range in scope from the development of novel techniques to assess in situ microbial biomass, activity and growth, to comprehensive field studies designed to elucidate the mechanisms and rates of microbiological cycling of C, N and P.
We study fundamental colloid and surface science and its applications to areas of biomedical and biotechnological interest. In particular, we focus on the biophysical study of lung surfactant and other self-assembled phospholipid/protein monolayers/bilayers/multilayers; pulmonary toxicology of nanomaterials and nanoparticle-based pulmonary drug delivery; and the development of advanced surface tension and contact angle measurement methodologies. The techniques we use include Atomic Force Microscopy (AFM), Langmuir-Blodgett (LB) technique, and drop shape analysis. The goal of our study is to explore the fundamental nature of biocolloids and biointerfaces, and to apply this knowledge to biomedical and clinical practices through an interdisciplinary and translational approach.
Dr. Lau's laboratory is currently focused on the regulation of gap junctions by tyrosine protein kinases and novel, interacting cellular proteins. Gap junctions are one of four major types of intercellular junctions that are involved in establishing intercellular adhesion and communication. Gap junctions are unique because they enable the direct interchange of small molecules (<1000 daltons) between the cytoplasms of adjacent cells. This activity provides a form of intercellular signaling that is essential for the proper functioning of a variety of normal tissues.
Calcification of cardiovascular tissues occurs in a variety of pathological conditions, including vascular injury, renal failure, diabetes mellitus, atherosclerosis, and aging. Mineralization is multifactorial and results from abormal changes in the balance between activators and inhibitors of calcification. We and others have established a link between ABCC6 and the chronic calcification of pseudoxanthoma elasticum (PXE) in human and the dystrophic cardiac calcification phenotype (DCC) in mice. ABCC6 is primarily expressed in liver and the kidneys but not in connective tissues. Therefore, we use various mice models to elucidate the characteristics of this novel mineralization inhibitor pathway.
Research in my laboratory focuses on environmental biochemistry and biotechnology. Our current research projects are in areas of phytoremediation and bioremediation, metabolomics, marine pollution and toxicology, biochemical and molecular mechanisms of action of insecticides, antibody engineering and development of antibody-based assays, tephritid fruit fly control, parapheromones for tephritid fruit flies, bioactive natural product chemistry, analytical and environmental chemistry, environmental fate of pesticides and pollutants, and pesticide residue research for pesticide registration.
Dr. Linda Chang's research focuses on neurology, neuroimaging (MRI, MR spectroscopy, functional MRI, PET, SPECT), HIV, substance abuse (methamphetamine, marijuana, cocaine), and aging.
The primary research interest of the laboratory is the study of the role of diacylglycerol pathways in cancer. As part of the Natural Products & Cancer Biology Program, we also participate in the screening of natural products in the search of novel anticancer agents.
1) RasGRP1 and mouse skin carcinogenesis
Our studies are directed towards the investigation of RasGRP1 signaling in mouse keratinocytes and its participation in skin carcinogenesis utilizing both, knockout and transgenic mouse models. This project is supported by NCI.
2) RasGRP1 in human keratinocyte biology and transformation
We are investigating the role of RasGRP1 in human epidermal cells, utilizing 3-D organotypic cultures (skin reconstructs). This project is supported by Hawaii Community Foundation.
3) Role of RasGRP in angiogenesis
We are examining the participation of some of the RasGRP isoforms in the effect of diacylglycerol analogs in angiogenesis, using HUVEC (human umbilical vein endothelial cells) as our model. This project is supported by Department of Defense.
Research Interests and Ongoing Projects:
• Viral vectors and vector-mediated gene transfer and transgene expression in vitro and in vivo
• Novel gene therapy approaches for HIV-1 infection in the central nervous system l
• Marine compounds and their antiviral activities
• Test and production of transgenic shrimp strains with viral resistance
• New methods for enhanced monitoring costal water contamination using environmental pathogens as an indicator
The Harold L. Lyon Arboretum is a leader in the fields of conservation biology, Hawaiian ethnobotany and horticulture. On its 194 acres, Lyon Arboretum maintains a world renowned collection of more than 5,000 tropical plant species; supports Hawaii's horticultural and agriculture industries; and works to preserve and restore Hawaii's tropical forests. It is an active research facility and academic resource offering a wide range of programs to local and international communities including an innovative and ground-breaking tissue culture program for propagation of endangered native Hawaiian plants. With an abundant average rainfall of 165 inches, this tropical rainforest setting is an ideal location for growing an enormous diversity of plants. The plant collection features heliconias, gingers, aroids, bromeliads, native Hawaiian plants, and one of the largest palm collections found in a botanical garden.
The Marine Viral Ecology Laboratory (MarVEL) group is part of the Department of Oceanography in the School of Ocean and Earth Science and Technology (SOEST) at the University of Hawaii at Manoa. Our group investigates the ecology and diversity of microbial life in the sea, including human pathogens in coastal waters. We are particularly interested in the diversity of viruses and how viruses influence the ecology and evolution of plankton. A summary of some of our research interests and projects can be found here. Members of MarVEL are affiliated with the Center for Microbial Oceanography - Research and Education (C-MORE), and the Hawaii Ocean Observing System (HiOOS). Some of our previous work was carried out in affiliation with the Pacific Research Center for Marine Biomedicine (PRCMB).
We are interested in a broad range of problems associated with the cellular, molecular, and evolutionary basis of biological pattern formation. My lab utilizes a variety of molecular and "classical" techniques of microinjection, cell labeling, ablation, and transplantation, to address fundamental problems in developmental biology in a broad phylogenetic context.
My lab is currently focused in three major areas. The first area of interest is to understand the role of the early cleavage program in the segregation of developmental potential in a wide variety of animals which share a mode of embryogenesis known as spiral cleavage (e.g., molluscs, annelids, nemerteans, sipunculids, echiurans, and polyclad flatworms). Of particular interest is the mechanism by which dorsoventral polarity is established in members of different spiralian phyla. We are also interested in understanding the origins and significance of naturally evolved variations in the spiral cleavage program, such as modifications associated with the abandonment of larval development in order to develop directly to a miniature adult form (i.e., direct development). We consistently use intracellular cell lineage labeling and cell ablation techniques in a wide variety of species for many of our experiments.
The second area of interest is gaining an understanding of the relationship between radially symmetrical and bilaterally symmetrical metazoans. Current theory predicts that bilaterians are derived from a radially symmetrical stock, yet there is little evidence for how such a transition might have occurred. For example, what is the relationship of the oral-aboral axis of radially symmetrical forms to the anterior-posterior of bilaterians? How did the dorsoventral axis arise? Is there evidence for the origins of major cleavage patterns in bilaterian development (e.g., spiral and radial cleavage) in radially symmetrical forms? To what degree are the molecular events underlying morphological patterning and cell type specification conserved in this group of animals? We are investigating these and other aspects of early development in representatives of both anthozoan cnidarian and ctenophore embryos.
The third area of focus is to understand the evolution of biological novelties. These include the origins of the "middle" germ layer (mesoderm), the evolution of the nervous system in the Metazoa, and the evolution of unique cell types (cnidocytes, colloblasts, sensory cells, etc.). Many of these studies utilize cnidarians (the starlet sea anemone Nematostella vectensis) and ctenophores (the lobate Mnemiopsis leidyi), both of whose genomes have been sequenced. We continue to developed functional techniques to uncover conserved and novel molecular mechanisms underlying novel cell type formation.
Dr. Mau's research focuses on Native Hawaiian health disparities, diabetes, endocrinology and metabolism.
The Metabolomics Shared Resource (MSR) is occupying ~1,000 square feet of laboratory space. The lab space is dedicated to biological sample treatment, mass spectrometry analysis, data processing and analysis and molecular biology for cancer metabolism, metabolomics, drug metabolism, pharmacokinetics, and other bioanalytical studies.
MSR services offered include metabolite extraction from samples including serum, plasma, urine, and tumor tissues, metabolic profiling of human or animal samples, metabolomics data processing, analysis and interpretation.
The Microscopy and Imaging Core at the University of Hawaii Cancer Center (CC) provides access to a wide range of imaging instruments to UHCC member, and will soon extend the service to non-members. The facility also offers access to a licensed MetaMorph software package for the processing and analysis of microscopy images.
Currently, the core houses four major instruments and three microscopes for routine fluorescence and brightfield use.
The core is also in charge of maintenance and service of an IVIS Lumina (Caliper Life Sciences). This is a highly sensitive system to image fluorescent and/or bioluminescent reporters both in vivo and in vitro.
Assisted reproduction enables achieving fertilization when normal conception does not occur due to a variety of gamete defects. Intracytoplasmic sperm injection (ICSI) is the injection of a single spermatozoon directly into the cytoplasm of an oocyte using an injection pipette. This technique has been applied successfully in the treatment of infertile couples and is now widely used as a method of human assisted reproduction (ART). In addition to its obvious role in overcoming the infertility, ICSI is also an excellent tool allowing exploring new venues of reproductive biology. It provides a unique opportunity to examine the mechanisms underlying male infertility by looking into the reproductive potentials of individual spermatozoa.
The primary research interest of the lab is to study sperm genetics and function in fertilization in the context of assisted reproduction, utilizing advanced techniques of gamete and embryo micromanipulation combined with cytogenetic and molecular biology techniques, in a mouse model. The overall goal is to explore how the 'genetic composition' of sperm translates on its function in fertilization.
The ongoing projects involve:
(1) Reproducing subfertile and infertile mice with phenotypes that mimic various human male infertility syndromes to test for ART effects;
(2) Studying sperm DNA damage, its origin and consequences for fertilization and embryo development;
(3) Examining the function of the Y chromosome encoded genes in male fertility.
Dr. Nerurkar's research interests are:
• Traditional Hawaiian medicine
• Traditional Ayurvedic medicine
• Role of alternative medicines in ameliorating obesity, insulin resistance and hyperlipidemia (metabolic syndrome).
• Signal transduction pathways involved in insulin signaling, lipid metabolism and glucose metabolism.
• Mechanisms of mitochondrial toxicity associated with obesity, insulin resistance, diabetes and drug-induced hepatotoxicity.
• ER function in obesity, insulin resistance and hyperlipidemia (metabolic syndrome).
• Role of reactive oxygen species (ROS) and nuclear transcription factors in obesity, insulin resistance and hyperlipidemia (metabolic syndrome).
Research focuses on the regulation of the presynaptic nerve terminal:
- Beta amyloid (Aβ) regulation of presynaptic calcium
- Lipid rafts in Aβ regulation
- Nicotine regulation via presynaptic nicotinic receptors
- Calcium homeostasis
• Theories of behavior change
• Motivation to engage in health behaviors
• Exercise/physical activity
• Multiple health behavior change
• Childhood obesity
• General Population
Dr. Novotny's research focus is in ethnic differences in diet, physical activity and body size and composition, especially patterns of growth and development, using anthropometry and DXA. She and colleagues have shown that Asian adolescent girls carry more trunk to peripheral DXA body fat than white girls, and that birth weight and birth length are inversely related to this relationship. They have also developed a novel measure of breast density, using DXA, which they are examining in girls and their mothers; and they are building a breast cancer risk model for Pacific Islanders that will expand the Gail model with novel risk factors. She was recently awarded a multi-million dollar multi-institutional grant to prevent child obesity throughout the Pacific Region.
Nutrition Support Staff at CRCH provides dietary assessment information that will support diet-related cancer research studies.
The staff of the resource perform several services:
- Maintain a food composition table with information on up to 174 nutrients and food components for over 2300 foods.
- Maintain a supplement composition table for up to 217 nutrients and food components in over 3300 dietary supplements.
- Develop quantitative scannable food frequency questionnaires for use in epidemiologic studies and provide the nutrient composition data needed for the analysis of questionnaire responses.
- Review the accuracy of food records, dietary recalls, and dietary supplement questionnaires, and assist with data entry procedures.
- Provide training for project staff in appropriate dietary data collection methods.
- Assist with the design, analysis, and interpretation of dietary data for studies of diet and cancer.
The Office of Technology Transfer and Economic Development's mission is to help University of Hawai‘i (UH) researchers identify, protect and commercialize intellectual property. It aspires to be a leading Asia Pacific technology transfer office, promoting and transferring the University of Hawai‘i's research discoveries to industry for the benefit of humanity.
Dr. Okihiro's research interests are in the area of childhood obesity and early metabolic risk, especially among children in Hawai'i. She is also interested in the development of obesity and how this issue can be addressed from a clinical and community perspective.
"The Computer Network Support Facility delivers services, information and tools to enhance the scientific productivity of biomedical and bioscience investigators at the University of Hawaii Manoa. Our clients include researchers, administrators and staff in the Pacific Biosciences Research Center and those associated with NIH center grants in the John A. Burns School of Medicine and other departments. Because the majority of our funding comes from the University of Hawaii Research Centers in Minority Institutions Program, we emphasize the development of computer and network tools that are useful to RCMI investigators."(http://www5.pbrc.hawaii.edu/cnsf/index.html)
The Pacific Research Center for Marine Biomedicine (PRCMB) at the University of Hawaii, led by Edward Laws and Richard Yanagihara, is conducting interdisciplinary research on harmful algal blooms, water- and vector-borne diseases, and marine-derived pharmaceuticals and probes. Knowledge gained from this research will advance national health and lead to improved strategies to reduce the burden of human diseases resulting from acute and chronic exposures to risks in the ocean environment.
Dr. Panee's research focuses on the mechanism and evaluate translational application of natural products in the prevention and treatment of obesity-associated diseases and breast cancer.
Our current work is focused on determining how expression of cancer-associated genes c-myc and p53 affects sensitivity to TNF and FasL induced programmed cell death in cell lines representing various stages on the pathway to cancer. We are also using our cell lineages to determine whether activation of caspase-2 plays a role in the differential sensitivity of cells to TNF- and FasL-mediated cytotoxicity. In addition, studies are underway to characterize TNF- and FasL-induced changes in mitochondrial membranes. This involves monitor the appearance of mitochondrial-associated proteins involved programmed cell death such as caspase-2, caspase-9, cytochrome c, and apoptosis Inducing factor.
The Pathology Shared resource capitalizes on the existing infrastructure of the Center's tissue repository. The Repository currently consists of fixed, paraffin-embedded tissue specimens from hospitals and laboratories statewide. The purpose of the repository is to provide tissue specimens to Center members and other investigators for research purposes. A priority is placed on the provision of specimens for population-based research and peer-reviewed, extramurally funded projects. The repository is a part of the Residual Tissue Repository Program of the NCI SEER program (http://seer.cancer.gov/biospecimen/).
Our proteomics core facility provides state-of-the-art proteomics platforms, resources, expertise and training to advance clinical and biomedical research and education to the biomedical community of researchers in Hawaii on a cost-recovery basis. It is our goal to ensure that researchers have access to proteomics resources that will improve the outcome of their biomedical research and facilitate collaborations with investigators from other institutions across the country.
The RCMI Bioinformatics Facility at UHM gives local researchers access to computational resources (hardware and software), and offers bioinformatics support either as a service or as a collaboration. The core specializes in pipelines for the bioinformatics analysis of high-throughput sequencing and gene expression data.
The Greenwood Molecular Biology Facility is a primary research infrastructure operated by the Biotechnology Program of the Pacific Biosciences Research Center. The Facility is vital to the educational and research mission of the University of Hawaii by serving the biomedical and molecular biology research and educational programs of the University and its associated institutions. The activities of the the Facility are structured to address both the present and the future needs of researchers for services in genomics, proteomics and bioinformatics.
This lab provides routine histology processing, specialized histochemical and immunohistological techniques. The core also provides technical assistance, training, and consultation in histological techniques to investigators and students throughout the University on a cost-recovery basis.
This core facility provides access to several microscopic platforms. The core also provides technical assistance, training, and consultation in imaging techniques to investigators throughout the University. At present, all of the equipment is available to investigators for use after certified training. Use of the confocal microscopes has an hourly charge.
The Magnetic Resonance Imaging (MRI) Research Center is located at the Queen's Medical Center. It houses a 3 Tesla MRI Scanner that is dedicated to basic and clinical research. The RCMI MRI Core operates through the Queen's MRI Research Center to provide technical support and training for clinical investigators, in particular those at the junior level, and allow support of pilot studies and studies with new technical approaches to investigate diseases that disproportionately impact minority populations as health disparities.
We conduct microarray-related experiments, including developing chips for pathogen discovery, evaluating RNA quality and test chips, processing RNA specimens, hybridizing microarray chips, scanning microarray chips and providing raw data for analysis.
Our core provides a standardized library of pathogens and reagent materials.
This lab focuses on: 1 understanding the underlying mechanisms controlling cancer cell proliferation and invasion; 2 the role of PEA-15 and the ERK MAP kinase pathway in cancer formation and progression; 3 dysregulation of cell signaling in neuroblastoma and glioblastoma; 4 developing new drugs to target cancer based the lab's basic research findings.
Funding: NIH and DOD.
The Rappé laboratory for aquatic microbial ecology is located at the Hawaii Institute of Marine Biology (HIMB), a research institute in the School of Ocean and Earth Science and Technology (SOEST) of the University of Hawaii at Manoa. The HIMB is located on Coconut Island in Kaneohe Bay, about a 30 minute drive from the main university campus.
Using a variety of approaches (genomics, molecular biology, microbial oceanography, and traditional microbiology), research in the Rappé Lab focuses on the diversity, evolutionary history, ecology, and physiology of microorganisms that inhabit coastal and open ocean seawater, corals and coral reef environments, and the deep subsurface biosphere.
My laboratory studies the genetic and hormonal regulation of nervous system development in the fruit fly Drosophila melanogaster. We are particularly interested in understanding how developmental signals such as hormones, nuclear receptors, trophic factors and synaptic contacts regulate gene expression to control nervous system development and function.
One project in the lab focuses on the regulation of programmed cell death, or apoptosis, in the nervous system. In Drosophila, the genes reaper, grim and head involution defective (hid) induce apoptosis when expressed at high levels. We have identified two distinct sets of neurons that die by apoptosis shortly after the emergence of the adult from the pupal case at the conclusion of metamorphosis, in a low steroid hormone environment. Both sets of neurons accumulate reaper and grim transcripts, but not hid transcripts immediately prior to their death. Application of the biologically active steroid 20-hydroxyecdysone prevents transcription of these genes and prevents the death of these neurons. We have identified a genomic region that regulates expression of the grim gene that is sensitive to the titer of 20E. We are currently characterizing this region to determine the mechanism by which 20E represses transcription of this gene.
The steroid hormone 20E drives Drosophila development by interacting with nuclear receptors that act as ligand regulated transcription factors. To define the cellular mechanisms and interactions involved in nuclear receptor signaling, we are currently conducting a screen to identify genes that are involved in nuclear receptor signaling pathways. This screen is designed to identify genes that function during metamorphosis when the animal undergoes a dramatic reorganization. Characterization of genes identified in this screen will help define the molecular pathways that are regulated by hormones during development.
This is a relatively new lab. Funding: NSF, DARPA, National Geographic. Research focuses on 1 understanding the relationships between different groups of organisms at the population level, species levels, and above 2 building DNA and morphology-based phylogenies to identify new species and uncover hidden relationships and patterns between species. Current projects focus on the application of systematics to improve agriculture and conservation efforts in Hawai'i and elsewhere.
ESF project support comes under four categories:
1) New Funded Grants:
The ESF supports researchers in planning proposals that will utilize ESF capabilities. For funded grants, the support should ideally begin at the early proposal stage. In consultation with the researcher, ESF can suggest practical and creative ways of obtaining and recording the desired data.
2) Support for Ongoing Funded Programs:
The Engineering Support Facility has personnel and experience to repair and maintain laboratory instruments and equipment. In some cases, the existing laboratory instruments need some custom enhancements or adaptations for the particular science in view. ESF has experience going back over 35 years in custom modifications and enhancements to existing instruments.
3) Machine Shop:
The ESF has a well-equipped machine shop and an expert instrument maker.
4) Pressure Test Facilities:
The ESF operates a hydraulic pressure test vessel that can test pressure cases from 15 to 10,000 psia, with electronic feed-thru capability. The test vessel can accommodate objects up to 30 inches in outside diameter by 13 feet length.
The Flow Cytometry Facility, operated out of the School for Ocean, Earth Sciences and Technology at the University of Hawaii, provides state of the art instrumentation for analyzing and sorting particles. Flow cytometers detect particles based on their size (scatter) and fluorescence (auto or imparted by a dye). These particles can be enumerated or simply characterized as to their scatter and fluorescence characteristics. Multiple populations can be separated visually and if desired, physically, based on these properties. Flow cytometers work ideally with cells in the 0.2 - 200 µm diameter size range and cell concentrations as low as 1000 cells/ml.
SWOG (formerly the Southwest Oncology Group) is one of the largest cancer clinical trials cooperative groups in the United States. Funded largely by research grants from the National Cancer Institute, part of the National Institutes of Health, SWOG's mission is to improve the practice of medicine in preventing, detecting and treating cancer.
• Preterm Birth
• Genitourinary Infections in Pregnancy
• Viral Infections in Pregnancy
• Peridontal Disease in Pregnancy
• Thyroid Disease
• Preterm Premature Rupture of Membranes
• Role of Infection in Preterm Birth
The School of Ocean and Earth Science and Technology was established by the Board of Regents of the University of Hawai‘i in 1988 in recognition of the need to realign and further strengthen the excellent education and research resources available within the University. SOEST brings together four academic departments, three research institutes, several federal cooperative programs, and support facilities of the highest quality in the nation to meet challenges in the ocean, earth and planetary sciences and technologies.
Dr. Seifried's research focuses on macromolecular interactions, physical biochemistry, bioinformatics, microbial and human molecular genetics.
The ASGPB genome infrastructure currently consists of several state-of-the-art capillary-based DNA sequencers and accessory equipment to deal with large sample volumes. The facility can process up to 9000 samples per day with a projected sequence output of 6.75 Million bases. Several prokaryotic and one eukaryotic genome project are currently in progress.
The facility offers DNA sequencing services for a wide range of templates including plasmid DNA, PCR fragments, cosmid DNA, and Bacterial Artificial Chromosomes (BACs). Sequencing reactions are performed using Applied Biosystems BigDye terminator chemistry and are run on ABI 3730XL capillary-based DNA sequencers.
Dr. Shiramizu's research interests focus around the role of HIV-1 in causing neurological problems and cancer; and childhood cancers.
Researchers in the Shohet Laboratory are engaged in exploring the response of the stressed heart in mouse models.
Current Research focuses on:
1. HIF-1 Overexpression
2. Endothelial Responses to Cardiovascular Stress
3. Elucidating the Role of Endothelin-1 in the Heart
4. Ultrasound Targeted Microbubble Destruction (UTMD)
5. Human Genetics
Dr. Stewart’s lab specializes in Human Nutrition research. The lab’s research scope ranges from basic benchwork to clinical trials. Dietary fiber and intestinal health are the lab’s focuses. Specific topics of interest are:
*Dietary fiber content of local foods
*Fiber as a functional food ingredient
*Physiological effects of dietary fiber with an emphasis on fiber fermentation
*Prebiotic effect of dietary fiber
*Influence of gut microbiota on fiber fermentation
Researchers in the Stokes Lab are focused on exploring regulatory protein mechanisms in the stressed and failing heart, specifically, ion channels and complex trans-membrane proteins.
Dr. Taylor’s primary research is on malarial immunity, including identification of immune responses associated with protection and pathology. Early studies examined cellular and humoral immune responses to malaria using rodent and primate models. These studies lead to producing monoclonal antibodies (mAb) against Plasmodium falciparum, the parasite that causes human malaria. Since 1990 she has conducted research and training of graduate and doctoral students in Cameroon, receiving continued NIH support since 1994. More recently, Dr. Taylor began evaluating how malaria infections during pregnancy influence acquisition of immunity of their babies to malaria during the first year of life.
"The University of Hawaii Telehealth Research Institute (TRI) is a research group administered through the John A. Burns School of Medicine, Office of the Dean. The mission of the group is to conduct original work in the use of telecommunications and advanced computing in the delivery of medical care and medical education. The group serves as a resource for Hawaii and the academic community at large." --from the website
The University of Hawaii Biorepository is a non-commercial, NIH-sponsored, core facility that provides biomedical researchers restricted access to human biological samples and clinical data.
The UH Biorepository contains three resources: 1) The Comprehensive Human Organ and Tissue Bank, 2) The Human Reproductive Biospecimen Repository, and 3) The INBRE III Biorepository and in vivo Model Resource. See below for more details.
For instructions on how to apply for access to our samples, please visit: uhbio.jabsom.hawaii.edu.
Dr. Verma’s long-term research goal is to understand immunological events associated with pathogenesis of neurotropic viruses to ultimately design therapeutic interventions and/or adjunct therapies to improve disease pathology. Her research employs both, in vitro and in vivo mouse models to delineate various innate immune signaling pathways that contribute to antiviral defense and inflammation in flavivirus infections. The focus of her COBRE project is to analyze mechanisms associated with disruption of the blood-brain barrier and its consequence with respect to the entry of West Nile virus (WNV) in the mice brain. In addition, ongoing studies are investigating the efficacy of anti-inflammatory drugs such as inhibitors of matrix metalloproteinases and cyclooxygenase-2 signaling pathway as a potential therapeutic target to manage WNV encephalitis. Another area of her research is to understand the role of NLR family of pathogen recognition receptors, NLRP3, NLRC5 and inflammasome adapter molecule ASC in modulating innate and adaptive immune response to WNV. One of her recent project also involves characterizing the role of epigenetic modulation in innate immune responses to flaviviruses.
Our research is focused on the structure of mammalian sperm chromatin and how this is related to function. The main hypothesis that we have tested is that the sperm cell provided the newly developing embryo with more than just the genetic code in the DNA sequence; it also provides a three dimensional organization of the DNA that provides crucial information as to how to use the father's genetic code.
DNA is packaged very densely in the sperm nucleus in a manner that is different from any other cell type. Most of the histones are replaced by protamines, and the DNA is crystallized into dense toroids with roughly 50,000 bp of DNA, each. Protamine condensation protects the sperm DNA from damage from external insults, and prevents transcription or DNA replication from occurring. We have shown that one structural feature present in all other somatic cells is also retained in sperm chromatin: the organization of DNA into loop domains attached at their bases to the nuclear matrix. This organization is crucial to two aspects of sperm chromatin function. Shortly after fertilization, the protamines are removed from the sperm DNA and the chromatin is repackaged with histones. The DNA is then replicated, and we have demonstrated that this DNA synthesis requires the loop domain organization to be intact. On the other hand, spermatozoa have the ability to digest their own DNA through an apoptotic-like process in which the sperm DNA is degraded. This degradation occurs on the nuclear matrix.
Our current research efforts are focused on understanding how sperm chromatin structure is related to the events that occur shortly after fertilization and how the DNA packaging in the sperm cell contributes to embryonic development.
The Wellcome Trust Centre for Neuroimaging at UCL (incorporating the Leopold Muller Functional Imaging Laboratory and the Wellcome Department of Imaging Neuroscience) is an interdisciplinary centre for neuroimaging excellence.
The research interests of Dr. Williams center on the discovery and evaluation of these small molecule chemical defenses from marine sources as potential drug leads. In collaboration with the other members of the Cancer Biology Program, marine extracts are screened against a variety of relevant cancer targets. The active constituents are then isolated using a combination of bioassay data and repeated separations. The structures of these metabolites are then determined primarily through the use of high-field NMR spectroscopy and chemical degradation. For example, Dr. Williams and colleagues have recently begun searching for novel inhibitors of the Ras/ERK MAP kinase pathway. Constitutive activation of this pathway is commonly observed approximately 30% of all tumor types and in 90% of all pancreatic tumors in epithelial tumors, hence it represents a potential therapeutic target. To this end, they have begun assaying extracts derived from marine sponges and cyanobacteria in an effort to discover new structural classes of inhibitors. Efforts in the Williams lab are directed towards identifying and characterizing these active components.
Found 175 resource providers .