This is a summary list of all core laboratories at University of Hawai'i Manoa . The list includes links to more detailed information, which may also be found using the eagle-i search app.
The overall objective of the Animal Carcinogenesis Shared Resource is to provide the members of the Cancer Center with the ability to utilize animal carcinogenesis models in the implementation of their research projects. Animal in vivo experiments are required as a step of the processes of translational research by which the results of research done in the laboratory are used to develop new ways to diagnose and treat cancer.
A 2000 square foot BSL-3 facility located at the University of Hawaii, JABSOM.
"The Biological Electron Microscope Facility (BEMF) at the University of Hawai'i is a multi-user/service facility, administered by the Pacific Biosciences Research Center (PBRC). The mission of the BEMF is to provide biological-biomedical researchers with state-of-the-art instrumentation, training and services for high-resolution scanning electron microscopy, conventional and energy-filtering transmission electron microscopy, optical, fluorescence, and laser scanning confocal microscopy, and image analysis on a recharge basis. The facility receives partial support from the Pacific Biosciences Research Center and the UH Manoa Chancellor's Office."
Includes the Richard Allen Image Collection, a unique set of images taken with electron microscopes (http://www5.pbrc.hawaii.edu/allen/).
The University of Hawai’i John A. Burns School of Medicine Biostatistics & Data Management Core provides research design and biostatistical analysis collaborations and support to investigators from the John A. Burns School of Medicine, School of Nursing, as well as other schools and departments. The core serves as the biostatistics core/key function for several NIH institutional infrastructural grants at University of Hawaii, including RCMI Multidisciplinary And Translational Research Infrastructure eXpansion Program (RMATRIX), RCMI Bioscience Research Infrastructure Development for Grant Enhancement and Success Program (BRIDGES), RCMI Center for Native and Pacific Health Disparities Research (CNPHDR), and IDeA Networks of Biomedical Research Excellence (INBRE). Biostatistics services and collaborations include research design, data analysis, results dissemination, methodology development, and training and education. Biostatistical expertise covers epidemiologic investigations, bench science research, clinical studies and trials, and community-based investigations. Methodological research and the development of novel approaches are also conducted by this core. Consultations are provided to investigators and collaborative partnerships are formed to further develop biomedical research. This core also provides teaching and training in biostatistics and research design. Introductory and advanced biostatistics courses, research ethics courses, and seminars are offered to educate and foster growth in multidisciplinary and translational research. The biostatistics website also includes an online library of statistical tools and educational materials.
The Biostatistics Shared Resource (BSR) provides statistical consultation and support to the research enterprise of the University of Hawai‘i Cancer Center. The primary goal of the resource is to improve the quality of the research through sound statistical principles. The resource was established in 1995 and provides access to statistical support to all Center members on all aspects of research. The BSR provides short-term as well as long-term support for research projects. Support is provided to scientists in epidemiology, health behavior and quality of life research, and molecular and clinical oncology. The members of the Shared Resource often serve as co-investigators on epidemiology and cancer prevention and control projects. Consultation is available in the following areas: design, data collection, data analysis, statistical programming, interpretation and dissemination of results, and protocol review. The members of the resource have contributed to the publication of many peer-reviewed manuscripts from Center research projects. In addition, the statisticians work on methodological issues important to the research ongoing at UH Cancer Center. One area of interest is measurement error models that correct for bias in model parameter estimates when the exposure variable is measured with error. The role of dietary intake and sunlight exposure in cancer etiology are of interest at the Center, and these variables are measured with error. Another interest is in how susceptibility genes affect cancer risk, in combination with exposure data. Therefore, statistical techniques for the investigation of genes and lifestyle-gene interactions in cancer models are also studied.
The COBRE Bioinformatics Facility at UHM offers access to high speed computer hardware and bioinformatics consultation. Research at the facility includes studying of evolutionary change in emergent dengue viruses associated with increasingly severe epidemics and confirm the impact of these changes in experimental models.
Offers services in the areas of qPCR, Microarray, Sequencing, and Other Genomic Services. May expand or remove services depending on need or interest. If you have recurring processes that you would like to automate or have us take over, we would be glad to sit down and talk with you to discuss how we can help you acheive your goals. We are aiming to be a self-sustaining entity consisting solely on a chargeback system. Our prices reflect the costs of equipment service contracts, potential and actual maintenence costs, as well as direct and indirect labor costs and administration. Our costs are kept low by being able to provide services in bulk with high throughput, it is therefore sometimes necessary to have minimum orders or fees in order to maintain a positive cost structure.
The of the goal of the Immunology Core of MCI, which functions as a fee-for-service facility, is to provide the Hawaii community with a service-oriented facility to meet the needs in the FACS research areas. We are equipped with BD FACSAria, BD FACSCalibur, GuavaEAsyCytePlus, Luminex 200, Beckman-Coulter ViCell, CTL-ImmunoSpot® S5 Core Analyzer, Bio-Rad and BioTek ELx808 ELISA readers and washers, Faxitron compact 43855D x-ray irradiation system, and Faxitron x-ray CP160 for in vivo animal work.
"The Center for Cardiovascular Research provides a Mouse Phenotyping Core for the use of investigators at the University, and by special arrangement, for investigators anywhere in Hawaii. We can provide murine echocardiography, blood pressure determinations, surgical procedures and phlebotomy, as well as assistance with mouse husbandry and genotyping."(http://www2.jabsom.hawaii.edu/mousecore/)
"The Transgenic and Embryonic Stem Cell Gene Targeting Core is a state-of-the-art facility with the expertise in the production of genetically altered subjects. Transgenic subjects carrying new or novel genes are created by microinjection of DNA into the pronuclei of fertilized eggs. Knock-out lacking specific genes of interest are created by homologous recombination in embryonic stem cells followed by injection into blastocysts to create chimeric subjects. Highly experienced personnel produce transgenic and knock-out subjects for UH investigators at very reasonable cost and with very short lead times." (http://www.ibr.hawaii.edu/tc.html)
The Clinical Protocol and Data Management Shared Resource (CPDM) is a component of the Center’s Clinical Trials Unit that provides central management and oversight of all clinical trials activities of the Center.
The Shared Resource provides a range of management and quality control functions that are essential for conducting clinical trials research in Hawaii’s decentralized community practice setting. It includes a central location for all cancer protocols, a centralized database of protocol-specific data, an updated list of currently active protocols for the use of Center investigators, and status reports of protocols. It provides a centralized organization for activating and monitoring protocols throughout Hawaii’s multiple community offices and medical centers involving several Institutional Review Boards (IRBs). Quality control functions include: insuring subject safety and compliance with federal requirements through the Data and Safety Monitoring plan, insuring staff competency through training for research nurses, CRAs, clinical trials assistants and community office, clinic, and hospital staff engaged in Hawaii’s cancer clinical trials enterprise, and insuring recruitment of underserved minorities through focused outreach activities.
The Evolutionary Genetics Core Facility (EGCF) at the Hawaii Institute of Marine Biology (HIMB) is located on Coconut Island in Kaneohe Bay, Oahu. The NSF EPSCoR-supported Core Facility provides technical services and support for the faculty and students doing research at HIMB and the University of Hawaii system, as well as other institutions worldwide. The EGCF provides access to various instrumentation and equipment and offers fee-based genomic services including sequence and fragment analysis. The EGCF manager is available to provide services and assist with questions regarding lab instrumentation or protocols.
The purpose of the Genomics Shared Resource (GSR) laboratory is to provide expertise in the fast developing area of genomic analysis. In response to a growing need for genotyping services by a number of UH Cancer Center investigators, this laboratory was converted into a UH Cancer Center Shared Resource in 1999. Its current mission is to provide nucleic acid extraction, genotyping and expression profiling services in support of the peer-reviewed and IRB-approved research conducted by UH Cancer Center members.
HURL's facilities include two deep-diving (2000 m) submersibles Pisces V and Pisces IV, a remotely operated vehicle RCV-150, and the support ship R/V Ka'imikai-o-Kanaloa. The two submersibles are housed and maintained at Makai Pier.
HURL Data Archive: HURL provides a data package for each Pisces IV, Pisces V or RCV-150 dive. This package includes voice transcripts (Pisces IV & V only), graphs of environmental data (Pisces IV & V only), copies of videotapes with videologs, and copies of up to 250 slides with a photolog. The Data Archive is the custodian of the original data which remain the property of HURL. Databases of organisms and substrates observed on the dives are available for use by the principal investigator. Use of dive data by other scientists is subject to principal investigator approval for 2 years. HURL and NOAA-NURP reserve the right to use slides and video from dives for program promotion.
HSFL was established in May 2007 within the School of Ocean and Earth Science and Technology (SOEST) and the College of Engineering (CoE) at the University of Hawaii. As a multidisciplinary research and education center HSFL brings together individuals from diverse areas to work on the exploration and understanding of the space environment. The mission of HSFL is to:
* Promote innovative engineering and science research for terrestrial and planetary space missions.
* Develop, launch, and operate small spacecraft from the Hawaiian Islands to accelerate the validation of new space technologies.
* Provide workforce training in all aspects of unmanned space missions.
* Build synergistic collaborations among educational, governmental, and corporate institutions interested in space exploration.
Hawaii is located in a unique position to become a low-cost gateway to space and to place the University of Hawaii as the only university in the world to have both satellite fabrication capabilities and unique, direct access to orbital space. This will enable diverse missions that study Earth's oceans and continents from low-Earth orbit, as well as the testing of engineering experiments in the hostile environment of space.
The goals of the Bioinformatics Core are:
* To increase the research capacity in bioinformatics in the State of Hawaii
* Provide hardware, access, and support to researchers who utilize genomic and proteomic data
* Provide a web-based gateway with access to a variety of bioinformatics applications, including available proprietary software such as Celera, and support for this software
* Undertake collaborative project with INBRE researchers to develop novel tools or applications that address specific needs
* Develop an academic program in bioinformatics with the John A. Burns School of Medicine (JABSOM)
* Recruit a full-time junior faculty member to lead the academic program
* Conduct training workshops and courses, and run a user support helpdesk
The Informatics Shared Resource (ISR) of the Cancer Research Center of Hawaii (CRCH) was established as a core facility in 2004. Its goal is to promote multidisciplinary collaboration, and enhance the research excellence and productivity of CRCH by providing access to biomedical informatics expertise and computational support to all members of the Center. The prime objective of the ISR is to facilitate the management, sharing, and integration of diverse data types in cancer research, as well as the synthesis and analysis of more focused data sets from the basic, clinical, genomics and population sciences. The bioinformatics group of the ISR supports a dedicated scientific computing network for computational cancer biology,and leads the development and application of advanced algorithms and software tools that enable Center investigators to take a more integrative approach to cancer research for the purpose of accelerating the translation of basic research results into the clinic.
The University of Hawai'i Insect Museum serves as a center for insect identification and systematics-based research. We have workspace, equipment, and resources for detailed taxonomic analysis, and researchers from many different laboratories are able to work and interact under its auspices.
Laboratory Support supplies support services for laboratory-based programs at the University of Hawai‘i Cancer Center. This resource is operated in order to streamline and make more centralized common laboratory needs.
The Harold L. Lyon Arboretum is a leader in the fields of conservation biology, Hawaiian ethnobotany and horticulture. On its 194 acres, Lyon Arboretum maintains a world renowned collection of more than 5,000 tropical plant species; supports Hawaii's horticultural and agriculture industries; and works to preserve and restore Hawaii's tropical forests. It is an active research facility and academic resource offering a wide range of programs to local and international communities including an innovative and ground-breaking tissue culture program for propagation of endangered native Hawaiian plants. With an abundant average rainfall of 165 inches, this tropical rainforest setting is an ideal location for growing an enormous diversity of plants. The plant collection features heliconias, gingers, aroids, bromeliads, native Hawaiian plants, and one of the largest palm collections found in a botanical garden.
The Microscopy and Imaging Core at the University of Hawaii Cancer Center (CC) provides access to a wide range of imaging instruments to UHCC member, and will soon extend the service to non-members. The facility also offers access to a licensed MetaMorph software package for the processing and analysis of microscopy images.
Currently, the core houses four major instruments and three microscopes for routine fluorescence and brightfield use.
The core is also in charge of maintenance and service of an IVIS Lumina (Caliper Life Sciences). This is a highly sensitive system to image fluorescent and/or bioluminescent reporters both in vivo and in vitro.
Nutrition Support Staff at CRCH provides dietary assessment information that will support diet-related cancer research studies.
The staff of the resource perform several services:
- Maintain a food composition table with information on up to 174 nutrients and food components for over 2300 foods.
- Maintain a supplement composition table for up to 217 nutrients and food components in over 3300 dietary supplements.
- Develop quantitative scannable food frequency questionnaires for use in epidemiologic studies and provide the nutrient composition data needed for the analysis of questionnaire responses.
- Review the accuracy of food records, dietary recalls, and dietary supplement questionnaires, and assist with data entry procedures.
- Provide training for project staff in appropriate dietary data collection methods.
- Assist with the design, analysis, and interpretation of dietary data for studies of diet and cancer.
"The Computer Network Support Facility delivers services, information and tools to enhance the scientific productivity of biomedical and bioscience investigators at the University of Hawaii Manoa. Our clients include researchers, administrators and staff in the Pacific Biosciences Research Center and those associated with NIH center grants in the John A. Burns School of Medicine and other departments. Because the majority of our funding comes from the University of Hawaii Research Centers in Minority Institutions Program, we emphasize the development of computer and network tools that are useful to RCMI investigators."(http://www5.pbrc.hawaii.edu/cnsf/index.html)
The Pathology Shared resource capitalizes on the existing infrastructure of the Center's tissue repository. The Repository currently consists of fixed, paraffin-embedded tissue specimens from hospitals and laboratories statewide. The purpose of the repository is to provide tissue specimens to Center members and other investigators for research purposes. A priority is placed on the provision of specimens for population-based research and peer-reviewed, extramurally funded projects. The repository is a part of the Residual Tissue Repository Program of the NCI SEER program (http://seer.cancer.gov/biospecimen/).
Our proteomics core facility provides state-of-the-art proteomics platforms, resources, expertise and training to advance clinical and biomedical research and education to the biomedical community of researchers in Hawaii on a cost-recovery basis. It is our goal to ensure that researchers have access to proteomics resources that will improve the outcome of their biomedical research and facilitate collaborations with investigators from other institutions across the country.
The RCMI Bioinformatics Facility at UHM gives local researchers access to computational resources (hardware and software), and offers bioinformatics support either as a service or as a collaboration. The core specializes in pipelines for the bioinformatics analysis of high-throughput sequencing and gene expression data.
The Greenwood Molecular Biology Facility is a primary research infrastructure operated by the Biotechnology Program of the Pacific Biosciences Research Center. The Facility is vital to the educational and research mission of the University of Hawaii by serving the biomedical and molecular biology research and educational programs of the University and its associated institutions. The activities of the the Facility are structured to address both the present and the future needs of researchers for services in genomics, proteomics and bioinformatics.
This lab provides routine histology processing, specialized histochemical and immunohistological techniques. The core also provides technical assistance, training, and consultation in histological techniques to investigators and students throughout the University on a cost-recovery basis.
This core facility provides access to several microscopic platforms. The core also provides technical assistance, training, and consultation in imaging techniques to investigators throughout the University. At present, all of the equipment is available to investigators for use after certified training. Use of the confocal microscopes has an hourly charge.
The Magnetic Resonance Imaging (MRI) Research Center is located at the Queen's Medical Center. It houses a 3 Tesla MRI Scanner that is dedicated to basic and clinical research. The RCMI MRI Core operates through the Queen's MRI Research Center to provide technical support and training for clinical investigators, in particular those at the junior level, and allow support of pilot studies and studies with new technical approaches to investigate diseases that disproportionately impact minority populations as health disparities.
We conduct microarray-related experiments, including developing chips for pathogen discovery, evaluating RNA quality and test chips, processing RNA specimens, hybridizing microarray chips, scanning microarray chips and providing raw data for analysis.
Our core provides a standardized library of pathogens and reagent materials.
ESF project support comes under four categories:
1) New Funded Grants:
The ESF supports researchers in planning proposals that will utilize ESF capabilities. For funded grants, the support should ideally begin at the early proposal stage. In consultation with the researcher, ESF can suggest practical and creative ways of obtaining and recording the desired data.
2) Support for Ongoing Funded Programs:
The Engineering Support Facility has personnel and experience to repair and maintain laboratory instruments and equipment. In some cases, the existing laboratory instruments need some custom enhancements or adaptations for the particular science in view. ESF has experience going back over 35 years in custom modifications and enhancements to existing instruments.
3) Machine Shop:
The ESF has a well-equipped machine shop and an expert instrument maker.
4) Pressure Test Facilities:
The ESF operates a hydraulic pressure test vessel that can test pressure cases from 15 to 10,000 psia, with electronic feed-thru capability. The test vessel can accommodate objects up to 30 inches in outside diameter by 13 feet length.
The Flow Cytometry Facility, operated out of the School for Ocean, Earth Sciences and Technology at the University of Hawaii, provides state of the art instrumentation for analyzing and sorting particles. Flow cytometers detect particles based on their size (scatter) and fluorescence (auto or imparted by a dye). These particles can be enumerated or simply characterized as to their scatter and fluorescence characteristics. Multiple populations can be separated visually and if desired, physically, based on these properties. Flow cytometers work ideally with cells in the 0.2 - 200 µm diameter size range and cell concentrations as low as 1000 cells/ml.
The ASGPB genome infrastructure currently consists of several state-of-the-art capillary-based DNA sequencers and accessory equipment to deal with large sample volumes. The facility can process up to 9000 samples per day with a projected sequence output of 6.75 Million bases. Several prokaryotic and one eukaryotic genome project are currently in progress.
The facility offers DNA sequencing services for a wide range of templates including plasmid DNA, PCR fragments, cosmid DNA, and Bacterial Artificial Chromosomes (BACs). Sequencing reactions are performed using Applied Biosystems BigDye terminator chemistry and are run on ABI 3730XL capillary-based DNA sequencers.
The University of Hawaii Biorepository is a non-commercial, NIH-sponsored, core facility that provides biomedical researchers restricted access to human biological samples and clinical data.
The UH Biorepository contains three resources: 1) The Comprehensive Human Organ and Tissue Bank, 2) The Human Reproductive Biospecimen Repository, and 3) The INBRE III Biorepository and in vivo Model Resource. See below for more details.
For instructions on how to apply for access to our samples, please visit: uhbio.jabsom.hawaii.edu.
Found 39 core laboratories .