Proteomics Core Facility

Directors: Li, Qing X., Ph.D.; Li, Yihan, Ph.D.

Summary:

Our proteomics core facility provides state-of-the-art proteomics platforms, resources, expertise and training to advance clinical and biomedical research and education to the biomedical community of researchers in Hawaii on a cost-recovery basis. It is our goal to ensure that researchers have access to proteomics resources that will improve the outcome of their biomedical research and facilitate collaborations with investigators from other institutions across the country.

Affiliations:

• John A. Burns School of Medicine

People:

• Director: Li, Qing X., Ph.D.
  
• Director: Li, Yihan, Ph.D.
  

Resources:

Instruments

• BioRad 2D gel apparatus ( 2D gel electrophoresis apparatus )
  

• Bruker ImagePrep MALDI-imaging sample preparation station ( Sample preparation system )
  

• Bruker Michrom Advance Nano LC interfaced with amaZon Speed CaptiveSpray ETD ion trap MS system ( Ion trap mass spectrometer )
  

  Capabilities: Analysis of protein post-translational modifications, protein sequencing, protein profiling and identification.

• Bruker Michrom Advance Nano LC interfaced with maXis Impact CaptiveSpray Q-TOF MS system ( Time-of-flight secondary ion mass spectrometer )
  

  Capabilities: High resolution protein identification, bottom-up proteomics, top-down protein sequencing, pesticide screening, metabolomics, quantitative proteomics with SILAC, N15 or iTRAQ, glycosylation analysis, molecular mass calculation with sub-ppm accuracy.

• Bruker UltraflexIII TOF-TOF ( MALDI-TOF mass spectrometer )
  

  Capabilities: Protein identification, Top-down protein sequencing, LC-MALDI-TOF and TOF-TOF, quantitative proteomics, MALDI Imaging, MALDI Biotyper.
  
  Our MALDI-TOF is coupled to a LC-Proteineer fc II, for automated fraction collection and proteomics workflow.

• GE 2D gel ImageScanner III and IMP 7 1 concurrent network license ( Densitometer )
  

• License for local MASCOT server ( Computer server )
  

Protocols

• Basic guidelines – UH Proteomics Core ( Protocol )
  

• Data Analysis – maXis Impact ( Protocol )
  

• In-gel digestion protocol for MALDI-MS ( Protocol )
  

• In-solution trypsin digestion ( Protocol )
  

• Nano-LC with ion-trap analysis ( Protocol )
  

• Peptide mass fingerprint by MALDI workflow ( Protocol )
  

• Phosphopeptide enrichment ( Protocol )
  

• Potential interference and contaminant ions in MS ( Protocol )
  

• Protein sample preparation for nano-LC/MS ( Protocol )
  

• Sample preparation guidelines for protein identification from a gel ( Protocol )
  

Services

• Glycoprotein and phosphoprotein analysis ( Material analysis service )
  

• High resolution protein identification ( Material analysis service )
  

• Molecular mass determination ( Material analysis service )
  

• Protein sequencing ( Material analysis service )
  

Software

• BioTools ( Software )
  

  Peptide/Protein Data Analysis Package.

• BioTyper ( Software )
  

  Microorganism Identification and Classification.

• ClinProTools ( Software )
  

  Software for Biomarker Detection and Evaluation.

• Ingenuity Pathways Analysis ( Software )
  

• MetaboliteTools ( Software )
  

  Software for Metabolite Prediction and Identification.

• ProfileAnalysis ( Software )
  

  Software for profiling and statistical evaluation of LC/MS data sets.

• ProteinScape ( Software )
  

  Database System for Proteomics Project Management.

• RapiDeNovo ( Software )
  

Web Links:

• http://blog.hawaii.edu/proteomics/