Pathology Shared Resources

Director: Hernandez, Brenda, Ph.D.

Summary:

The Pathology Shared resource capitalizes on the existing infrastructure of the Center's tissue repository. The Repository currently consists of fixed, paraffin-embedded tissue specimens from hospitals and laboratories statewide. The purpose of the repository is to provide tissue specimens to Center members and other investigators for research purposes. A priority is placed on the provision of specimens for population-based research and peer-reviewed, extramurally funded projects. The repository is a part of the Residual Tissue Repository Program of the NCI SEER program (http://seer.cancer.gov/biospecimen/).

Affiliations:

• University of Hawai'i Cancer Center

People:

• Director: Hernandez, Brenda, Ph.D.
  

Resources:

Instruments

• ABI GeneAmp 9700 96-well PCR system ( PCR instrument )
  

  "GeneAmp® PCR System 9700 is specifically designed for the amplification of nucleic acids. The GeneAmp® PCR System 9700 consists of a base module and one of many interchangeable sample block modules. The 96-well GeneAmp® PCR System 9700 is designed for use with 0.2 ml reaction tubes or 96-well reaction plates for all of your routine PCR applications." Lab contains two of these instruments.

• Beckman Coulter Allegra X-12R centrifuge ( Refrigerated centrifuge )
  

  "Whether harvesting cells or separating blood components, the Allegra X-12 Series will simplify your processes and save you time. Offering maximum capacity, this centrifuge is ideal for streamlining general separations. Perfect for clearing debris, Isolating mononuclear cells and pelleting mammalian cells." With rotor swinging bucket. Instrument shared by Pathology Shared Resources and Hernandez Lab.

• Beecher tissue arrayer ( Tissue microarrayer )
  

  Lab contains two of these.

• Dako automatic staining machine ( Automatic staining machine )
  

• Leica microtome ( Microtome )
  

• Leica tissue processor ( Automatic tissue processor )
  

• Linear stainer ( Automatic staining machine )
  

  Instrument shared by Pathology Shared Resources and Hernandez Lab.

• Microplate luminometer ( Plate luminometer )
  

  Instrument is shared with the Hernandez Lab.

• Nikon microscope ( Microscope )
  

Services

• DNA extraction service ( Material processing service )
  

• Genotyping service ( Material analysis service )
  

• HPV testing service ( Material analysis service )
  

• Immunohistochemistry service ( Material analysis service )
  

• In situ hybridization service ( Material analysis service )
  

• Pathology consultation service ( Access service )
  

• Tissue embedding service ( Material processing service )
  

• Tissue microarray slides service ( Material processing service )
  

• Tissue microarrays service ( Material processing service )
  

• Tissue processing service ( Material processing service )
  

• Tissue sectioning service ( Material processing service )
  

• Tissue staining service ( Material processing service )
  

Web Links:

• http://www.uhcancercenter.org/index.php/research/shared-resources/pathology