Li Lab

Affiliations:

• College of Natural Sciences, University of Hawaii at Manoa
• Department of Microbiology, University of Hawaii at Manoa

People:

• PI: Li, Hongwei
  

Resources:

Instruments

• Beckman Coulter J2-21M centrifuge ( Refrigerated centrifuge )
  

• Bio-Rad gel documentation system ( Gel imaging system )
  

• Biotek microplate reader ( Microplate reader )
  

• Eppendorf AG 5804R centrifuge ( Refrigerated centrifuge )
  

• Eppendorf AG BioPhotometer ( Spectrophotometer )
  

  "State-of-the-art technology with an easy-to-use format in a compact footprint: that is the Eppendorf BioPhotometer! It's large key pads, pre-programmed methods, and pre-set wavelengths and conversion factors greatly simplify the sample quantification. The large LCD screen displays the sample concentrations, absorption values, OD260/OD230 and OD260/OD230 and sample dilutions at a glance."

• Fisher Scientific Accumet AB15 pH meter ( pH meter )
  

• Isotemp hybridization oven ( Hybridization oven )
  

• Mettler Toledo balance ( Analytical balance )
  

• Micro ultrasonic cell disruptor ( Ultrasonic cell disruptor )
  

• Millipore Corporation Synergy water purification system ( Water purification system )
  

• New Brunswick Scientific Excella E24 incubator shaker ( Incubator shaker )
  

• NuAire biosafety cabinet ( Biological safety cabinet )
  

• Precision Napco CO2 incubator ( CO2 incubator )
  

• Refrigerated incubator ( Incubator )
  

• Refrigerated waterbath ( Water bath )
  

• SPECTRO Analytical Instruments GmbH UV crosslinker ( UV crosslinker )
  

• Thermo Fisher thermal cycler ( PCR instrument )
  

• Thermolyne cryo biological storage system ( Cell storage device )
  

Reagents

• 4a-2s4 ( Cell line )
  

  Derived from anopheles gambiae larvae.

• Aag2 ( Immortalized embryonic cell line )
  

• AD293 ( Immortalized embryonic cell line )
  

• ATC-10 ( Cell line )
  

• BHK-21 ( Primary fibroblast cell line )
  

  Syrian hamster kidney fibroblast, PHLS.

• C6/36 ( Cell line )
  

• HEK-293 ( Immortalized embryonic cell line )
  

• HEK-293T ( Immortalized embryonic cell line )
  

• HMVEC ( Cell line )
  

  Normal human neonatal foreskin or adult skin capillaries.

• HUVEC ( Cell line )
  

• NIH-3T3 ( Cell line )
  

  An aneuploid murine cell line with a morphology resembling fibroblasts.

• pBac/DS ( Plasmid )
  

• pBac/DS-eGFP ( Plasmid )
  

• pBac/DS-Rluc ( Plasmid )
  

• pBac/eGFP ( Plasmid )
  

  Expresses marker eGFP.

• pBacT7/DS ( Plasmid )
  

• pBacT7/DS-eGFP ( Plasmid )
  

• pBacT7/DS-Rluc ( Plasmid )
  

• pcDNA3.1/eGFP-NS1 ( Plasmid )
  

• pcDNA3.1/ENS1 ( Plasmid )
  

• pcDNA3.1/ENS1-FLAG ( Plasmid )
  

• pcDNA3.1/ENS1eGFP-HA ( Plasmid )
  

• pcDNA3.1/FLAG-NS1 ( Plasmid )
  

• pcDNA3.1/NS1 ( Plasmid )
  

• pDS ( Plasmid )
  

• pDS-eGFP ( Plasmid )
  

• pDS-Rluc ( Plasmid )
  

• pET28a/NS1 ( Plasmid )
  

• pGL-Hygro/T7 ( Plasmid )
  

• pGL/T7 ( Plasmid )
  

• pGL/T7-Hygro ( Plasmid )
  

• pIcam-Rluc ( Plasmid )
  

• pIZ/ENS1 ( Plasmid )
  

• pUC/DS ( Plasmid )
  

• pUC/DS-eGFP ( Plasmid )
  

• pUC/DS-Rluc ( Plasmid )
  

• pVcam-Rluc ( Plasmid )
  

• RAW 264.7 ( Tumor-derived cell line )
  

  Mouse leukaemic monocyte macrophage cell line.

• S2 cell line ( Immortalized embryonic cell line )
  

• Sf21 ( Cell line )
  

  Pupal ovarian tissue of the Fall Armyworm Spodoptera frugiperda.

• THP-1 ( Tumor-derived cell line )
  

  Human acute monocytic leukemia cell line.

• Vero ( Cell line )
  

  African green monkey kidney epithelial cells.

Web Links:

• http://www.hawaii.edu/microbiology/Li/index.html