Ramos Lab

Summary:

This lab focuses on: 1 understanding the underlying mechanisms controlling cancer cell proliferation and invasion; 2 the role of PEA-15 and the ERK MAP kinase pathway in cancer formation and progression; 3 dysregulation of cell signaling in neuroblastoma and glioblastoma; 4 developing new drugs to target cancer based the lab's basic research findings.

Funding: NIH and DOD.

People:

• PI: Ramos, Joe, Ph.D.
  

Resources:

Instruments

• BD FACScan flow cytometer ( Flow cytometer )
  

  Has the most current software version of CellQuest.

• Eppendorf AG Mastercycler Gradient ( PCR instrument )
  

• New Brunswick Scientific C25 incubator shaker ( Incubator shaker )
  

  This is a floor incubator; incubator features a 4 liters flasks adapter for large bacteria growth.

• VWR 2425 CO2 incubator ( CO2 incubator )
  

• Zeiss Axiovert 200M inverted fluorescence microscope ( Fluorescence microscope )
  

  It comes with deconvolution software capabilities: FRET and FRAT.

Protocols

• Integrin activation assay protocol ( Protocol )
  

  Protocol is used to measure the activation state of the integrin alpha5 beta1 on the surface of the protein.

Reagents

• 3FN9.11 ( Protein reagent )
  

  Synthetic fibronectin.

Web Links:

• http://www.crch.org/Faculty/jramos/