Analytical Biochemistry Shared Resource

Summary:

The Analytical Biochemistry resource was established to support and facilitate collaborative cancer research between the various disciplines at the Center by providing chemical analyses which contribute to understanding chronic diseases in our population. It also assists in the determination of the nature or structure of biological molecules as well as the quantification of those compounds. The facility is equipped with essential instruments needed to perform analytical chemistry, and modern, state-of-the-art technology for difficult-to-measure and ultra-low concentrated compounds.

Affiliations:

• University of Hawai'i Cancer Center
• University of Hawaii at Manoa Organized Research Units

People:

• PI: Franke, Adrian, Ph.D.
  

Resources:

Instruments

• Multiplex amino assay system ( Flow cytometer analyzer )
  

• Roche Mira Plus CC chemistry analyzer ( Amino acid analyzer )
  

• Thermo Fisher computer ( Computer )
  

• Thermo Scientific Exactive LC tandem mass spectrometer with accessories ( Mass spectrometer )
  

• Thermo Scientific TSQ Ultra Quantum LC tandem mass spectrometer with accessories ( Mass spectrometer )
  

• Ultra high pressure liquid chromatography system ( Liquid chromatography column )
  

Services

• 1-hydroxy-pyrene in urine analysis service ( Material analysis service )
  

• 25 Hydroxy Vitamin D analysis service ( Material analysis service )
  

• 5-Nitro-gamma tocopherol in plasma analysis service ( Material analysis service )
  

• Adiponectin analysis service ( Material analysis service )
  

• Ammonia in urine analysis service ( Material analysis service )
  

• Apo-A1 in plasma by AA service ( Material analysis service )
  

• Apo-B in plasma by AA service ( Material analysis service )
  

• Apo-E in plasma by AA service ( Material analysis service )
  

• Caffeine and its metabolites in urine analysis service ( Material analysis service )
  

• Calcium in plasma by AA service ( Material analysis service )
  

• Coenzyme Q10 in plasma analysis by HPLC/ECD (oxidized, reduced and total Q10) ( Material analysis service )
  

• Creatinine in urine by AA service ( Material analysis service )
  

• CRP in plasma by AA service ( Material analysis service )
  

• DNA analysis by HPLC ( Material analysis service )
  

• DNA methylation, global (5-methyl-dC/dG) analysis service ( Material analysis service )
  

• ELISA-based assay service ( Material analysis service )
  

• Fatty acids in plasma or RBC's analysis service ( Material analysis service )
  

• Haptoglobin in plasma by AA service ( Material analysis service )
  

• HDL-cholesterol in plasma by AA service ( Material analysis service )
  

• Homocysteine in plasma analysis service ( Material analysis service )
  

• Interleuken-6 soluble receptor analysis service ( Material analysis service )
  

• Interleukin-2 analysis service ( Material analysis service )
  

• Interleukin-6 analysis service ( Material analysis service )
  

• Leptin analysis service ( Material analysis service )
  

• Lipid panel in plasma by AA (Chol, HDLC, TGL) service ( Material analysis service )
  

• Lipid-phase micronutrients in plasma or foods analysis by HPLC/PDA/FD (12 carotenoids, 2 retinoids, 3 tocopherols) ( Material analysis service )
  

• Luminex based ELISA assay service ( Material analysis service )
  

• Metabolomics analysis by LCMS ( Material analysis service )
  

• Nitrogen and urea in urine by AA service ( Material analysis service )
  

• Parathyroid hormone analysis service ( Material analysis service )
  

• Phenolic agents from plasma, urine, breast milk, foods analysis by LC/MSMS ( Material analysis service )
  

  Isoflavonoids, flavonoids, lignans, anthocyanins or other groups of phenolic agents from plasma, urine, breast milk, foods by LC/MSMS service.

• Red blood cell preparation service ( Material processing service )
  

• Steroids in urine analysis service ( Material analysis service )
  

• TNF alpha analysis service ( Material analysis service )
  

• TNF alpha receptor I analysis service ( Material analysis service )
  

• TNF alpha receptor II analysis service ( Material analysis service )
  

• Total cholesterol in plasma by AA service ( Material analysis service )
  

• Total phenol levels in food, urine, and plasma analysis service ( Material analysis service )
  

• Triglycerides in plasma by AA service ( Material analysis service )
  

• Urinary isoprostane analysis service ( Material analysis service )
  

• Vitamin A and pro-vitamin A in plasma analysis by HPLC/PDA ( Material analysis service )
  

• Vitamin C in plasma, food analysis by HPLC/ECD or PDA ( Material analysis service )
  

• Vitamin D in plasma analysis by LC/MSMS ( Material analysis service )
  

  Applies to Vitamin D2, vitamin D3, 25-hydroxy-vitamin D2, and 25-hydroxy-vitamin D3.

• Vitamin E in plasma analysis by NP-HPLC/PDA/FD (all tocopherols) ( Material analysis service )
  

• Vitamin K analysis by LC/MSMS ( Material analysis service )
  

Web Links:

• http://www.uhcancercenter.org/research/shared-resources/analytical-biochemistry