Shohet Lab

Summary:

Researchers in the Shohet Laboratory are engaged in exploring the response of the stressed heart in mouse models.
Current Research focuses on:

1. HIF-1 Overexpression
2. Endothelial Responses to Cardiovascular Stress
3. Elucidating the Role of Endothelin-1 in the Heart
4. Ultrasound Targeted Microbubble Destruction (UTMD)
5. Human Genetics

Affiliations:

• Department of Medicine, John A. Burns School of Medicine

People:

• PI: Shohet, Ralph
  

Resources:

Instruments

• 384 real time PCR machine ( Real-time PCR machine )
  

• 96-well capillary sequencer ( Multicapillary electrophoresis system )
  

• Biomek robotic liquid handler ( Liquid handler )
  

• Columbus Instruments exercise system ( Animal physiology monitoring system )
  

  Shared with COBRE Mouse Phenotyping Core.
  
  Used to monitor cardiovascular activity in mice.

• Eppendorf AG 5417R centrifuge ( Refrigerated centrifuge )
  

• Hermle universal centrifuge ( Centrifuge )
  

• Hypoxia chamber ( Hypoxic chamber )
  

• IonOptix calcium detector ( Calcium detector )
  

• Langendorff perfusion system ( Perfusion station )
  

• Lonza Amaxa Nucleofector II device ( Nucleofector device )
  

  "The basic Nucleofector® II Device is a single cuvette based system that has been used in research labs since 2001. It allows efficient transfection of hard-to-transfect cell lines and primary cells with different substrates, e.g. DNA vectors or siRNA oligonucleotides, in low throughput format."

• Millipore water purification system ( Water purification system )
  

• Nanodrop spectrophotometer ( Spectrophotometer )
  

• Olympus microscope ( Inverted microscope )
  

Organisms and Viruses

• ETA 1 ( Mus musculus )
  

  This animal model is a knock-out model that focuses on cardio-specific diseases. Dr. Shohet has 3 animal models (strains) to study the endothelin system.

• inducable HIF 1 alpha ( Mus musculus )
  

  Tat-inducable oxygen stabilize cardiospecific HIF 1 alpha.

• PLUNC knockout ( Mus musculus )
  

Protocols

• FACS sorting for endothelial cells protocol ( Protocol )
  

Reagents

• HIF 1 alpha ppn ( Plasmid )
  

  This is a mutagenized plasmid that is resistant to oxygen.

• PLUNC expression plasmid ( Plasmid )
  

Services

• Invasive hemodynamics service ( Material analysis service )
  

• Mice surgery service ( Material modification service )
  

Web Links:

• https://sites.google.com/site/ccrheart/research/shohet-lab