Shikuma Laboratory

Affiliations:

• Department of Medicine, John A. Burns School of Medicine
• John A. Burns School of Medicine

People:

• PI: Shikuma, Cecilia
  

Resources:

Human Studies

• Pilot study of the efficacy and hepatic safety of raltegravir-based and efavirenz-based regimens when given in combination with tenofovir and emtricitabine in antiretroviral-naïve HIV-infected subjects co-infected with hepatitis C ( Clinical trial )
  

  An open-label, randomized clinical trial

• Thai Red Cross AIDS research study ( Clinical trial )
  

  The South-East Asia Research Collaboration Hawaii Consortium received several R1 grants from NIH to establish the Thai Red Cross AIDS Center in Bangkok, where a number of studies that focus on HIV infections take place.

Instruments

• Baker SG403 biological safety hood ( Laminar flow hood )
  

  This lab contains two biosafety cabinets.

• Beckman Coulter 746SY cell counter ( Automatic cytometer )
  

• Becton Dickinson FACSLoader/FACSCalibur sample carousel ( Sample loader )
  

• Bio-Rad thermal cycler/real-time PCR ( Thermal cycler )
  

• Biosafety cabinet with UV light ( Biological safety cabinet )
  

• GE Achilles Insight bone ultrasonometer ( Ultrasound machine )
  

• IEC Centra GP8R ( Centrifuge )
  

  The lab contains two of these instruments.

• Infoblox server ( Computer server )
  

• Labconco bench flow hood ( Laminar flow hood )
  

• Molecular Devices microplate reader ( Microplate reader )
  

• Molecular Devices Skanwasher plate washer ( Microplate washer )
  

• Perkin Elmer thermal cycler ( Thermal cycler )
  

• PerkinElmer Inc. GeneAmp PCR System 9700 ( Thermal cycler )
  

  "The GeneAmp 9700 is an automated instrument that is specfically designed for the amplification of nucleic acids using the Polymerase Chain Reaction (PCR) process. The user interface comes complete with a control panel with numeric keypad, soft keys, and a graphical digital screen that shows the time and temperature profile of each run. The heated lid and unique Thermal Isolation Frame ensure oil-free operation in the 0.5 mL format."

• Promega microplate luminometer ( Plate luminometer )
  

• Refrigerated benchtop centrifuge ( Refrigerated centrifuge )
  

• Roche refurbished lightcycler ( Real-time PCR machine )
  

• Safeaire biosafety cabinet ( Biological safety cabinet )
  

• Sorvall refrigerated tabletop centrifuge ( Refrigerated centrifuge )
  

• Sterimatic autoclave ( Autoclave )
  

• Turner lBiosystems uminometer with DLR assay kit ( Multimode microplate reader )