Patek Lab

Summary:

Our current work is focused on determining how expression of cancer-associated genes c-myc and p53 affects sensitivity to TNF and FasL induced programmed cell death in cell lines representing various stages on the pathway to cancer. We are also using our cell lineages to determine whether activation of caspase-2 plays a role in the differential sensitivity of cells to TNF- and FasL-mediated cytotoxicity. In addition, studies are underway to characterize TNF- and FasL-induced changes in mitochondrial membranes. This involves monitor the appearance of mitochondrial-associated proteins involved programmed cell death such as caspase-2, caspase-9, cytochrome c, and apoptosis Inducing factor.

Affiliations:

• Department of Microbiology, University of Hawaii at Manoa

People:

• PI: Patek, Paul, Ph.D.
  

Resources:

Instruments

• Beckman Coulter Allegra X-22R centrifuge ( Refrigerated centrifuge )
  

  "The compact Allegra X-22 Series is up to 10 inches/ 25 cm thinner than other benchtop centrifuges. With a library of 11 rotors, the Allegra X-22 Series is the space-saving multipurpose solution for your requirements."

• Beckman Coulter spectrophotometer ( Spectrophotometer )
  

• Bioflow fermenter ( Fermentor )
  

• Chromatograph ( Chromatography instrument )
  

• Gravity sterilizer ( Autoclave )
  

  Steam heated.

• Multifunction microplate reader ( Multimode microplate reader )
  

• Speedvac system ( Vacuum centrifuge concentrator )
  

Web Links:

• http://www2.hawaii.edu/~patek/