eagle-i University of Hawai'i ManoaUniversity of Hawai'i Manoa
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Genomics Shared Resource (GSR) Core Facility

Director: Le Marchand, Loic

Summary:

The purpose of the Genomics Shared Resource (GSR) laboratory is to provide expertise in the fast developing area of genomic analysis. In response to a growing need for genotyping services by a number of UH Cancer Center investigators, this laboratory was converted into a UH Cancer Center Shared Resource in 1999. Its current mission is to provide nucleic acid extraction, genotyping and expression profiling services in support of the peer-reviewed and IRB-approved research conducted by UH Cancer Center members.

Affiliations:

People:

Resources:

Instruments

  • ABI GeneAmp 9700 96-well PCR system ( PCR instrument )

    "GeneAmp® PCR System 9700 is specifically designed for the amplification of nucleic acids. The GeneAmp® PCR System 9700 consists of a base module and one of many interchangeable sample block modules. The 96-well GeneAmp® PCR System 9700 is designed for use with 0.2 ml reaction tubes or 96-well reaction plates for all of your routine PCR applications." This core facility has 11 PCR thermal cyclers.

  • ABI open array system ( Microarray platform )

  • ABI PCR thermocycler ( Thermal cycler )

  • Agilent Bioanalyzer 2100 ( Microfluidics platform )

    "The Agilent 2100 Bioanalyzer is a microfluidics-based platform for sizing, quantification and quality control of DNA, RNA, proteins and cells on a single platform. Results are delivered within 30-40 minutes in automated, high quality digital data."

  • GeneChip Scanner 3000 7G ( Array scanner )

    Includes scanner.

    "The GeneChip® Scanner 3000 7G is an extension of the GeneChip® Scanner 3000 (GCS 3000) series that allows you to scan next-generation higher-density arrays, including SNP arrays with up to 900,000 SNPs, tiling arrays for transcription and all-exon arrays for whole-genome analysis.

    The GCS 3000 7G combines space-saving and other design improvements with speed, superior performance and lower scanner-to-scanner variation to dramatically improve throughput and efficiency in your genetic analysis. The GCS 3000 7G also provides enhanced auto-focus and more consistent scanner-to-scanner biological performance to improve data integrity and data sharing between researchers."

  • HT Fast Real-Time PCR system ( Real-time PCR machine )

  • iScan array scanner ( Microarray platform )

  • NanoDrop spectrophotometer ( Spectrophotometer )

  • Plate replicator ( Dispensing well plate )

  • PyroMark Q24 sequencer ( DNA sequencer )

  • Qiagen Biorobot 3000 with Rapidplate 96 ( Nucleotide-free workbench )

  • Rapidplate 96 liquid handler ( Liquid handler )

  • SpectraMax Gemini XPS Fluorescence microplate reader ( Microplate reader )

    "The Gemini™ XPS Fluorescence Microplate Reader uses dual monochromators for variable wavelength selection between 250 nm and 850 nm to eliminate the need for searching out the right pair of excitation and emission filters. Dual monochromators also enable wavelength scanning across a range of wavelengths in increments as small as 1 nm to optimize assay parameters.

    The Gemini XPS Microplate Reader can measure up to 4 wavelength pairs for endpoint and kinetic measurements and offers well scanning to report a fluorescent measurement from a single point in the center of a microplate well to multiple points across a tissue culture well. Microplate sizes from 6- to 384-well can be read on the Gemini XPS Microplate Reader."

  • Transgenomic wave system ( High performance liquid chromatography instrument )

Services


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Last updated: 2011-10-20T14:14:59.730-05:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016