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|  University of Hawai'i Manoa |
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| University of Hawai'i Manoa |
The Hawai‘i Institute of Marine Biology is a world-renowned marine research institute of the School of Ocean & Earth Science & Technology (SOEST) at the University of Hawai'i at Manoa. Situated on Moku o Lo'e (Coconut Island) in Kane‘ohe Bay, HIMB provides excellent opportunities for tropical marine research located just 15 miles from the main campus and downtown Honolulu.
HIMB offers cutting edge research facilities for faculty, students, and visiting scientists coupled with convenient access to a diverse range of marine environments. For example, Coconut Island is surrounded by 64 acres of coral reef designated by the state of Hawai‘i for research activities only as the Hawai‘i Marine Laboratory Refuge. Research at HIMB covers many disciplines of tropical marine science such as coral ecology, biogeochemistry, and evolutionary genetics. In addition, HIMB faculty are recognized authorities in marine diseases, neuroendocrinology, microbial organisms, and sensory systems of marine mammals and elasmobranchs. Visit our Faculty and Research pages to learn more about ongoing scientific activities at HIMB.
Graduate and undergraduate students conducting research on Coconut Island are most often enrolled in the departments of Zoology and Oceanography at the University of Hawai’i, but also include students from a variety of departments and programs across campus such as the departments of Microbiology, Geography, Molecular Biosciences and Bioengineering (MBBE), Human Nutrition, Food and Animal Sciences (HNFAS), and the Global Environmental Sciences program at the department of Oceanography.
The Zeiss LSM710 laser-scanning confocal microscope is able to generate high-resolution images of fluorescent specimens. It is especially suited for live cell imaging of relatively thick samples (up to approx. 100 µM). Its scanning unit has a spectral recycling loop, two sensitive PMT detectors and a filter-free spectral separation module that can be continuously set over the entire wavelength range. It is equipped with lasers from violet to far red (405, 458, 488, 514, 561 and 633 nm excitation wavelengths). It has a motorized stage capable of tiling, multi-point mark, and find memory function. Current stage holders will support slides, circular glass-bottom culture dishes, and well plates with glass-coverslip bottoms. It is also equipped with an environmental chamber and full stage incubator for precise temperature and CO2 control. The purchase of the Zeiss LSM 710 confocal microscope for HIMB was made possible by a generous donation from Pam Omidyar (financing provided through the School of Ocean and Earth Science and Technology - SOEST).
The Zeiss LSM 710 Confocal Laser Scanning Microscope system has a multitude of functions for fluorescent imaging and analysis. Some of the features of this system include the following:
- Colocalization of fluorescent components
- Fluorescence intensity measurements for any region of interest
- Z-Sectioning of specimens for orthogonal & 3D analysis
- Time lapse imaging of dynamic processes in living specimens
- Fluorescence Resonance Energy Transfer (FRET)
- Fluorescence Recovery After Photobleaching (FRAP)
- Spectral imaging for the separation of overlapping emission spectra of specimens labeled with more than one fluorescent dye or expressing more than one innate fluorescent protein.
The purchase of the Zeiss LSM 710 confocal microscope for HIMB was made possible by a generous donation from Pam Omidyar (financing provided through the School of Ocean and Earth Science and Technology - SOEST).