Evolutionary Genetics Core Facility

Director: Eggers, Amy

Summary:

The Evolutionary Genetics Core Facility (EGCF) at the Hawaii Institute of Marine Biology (HIMB) is located on Coconut Island in Kaneohe Bay, Oahu. The NSF EPSCoR-supported Core Facility provides technical services and support for the faculty and students doing research at HIMB and the University of Hawaii system, as well as other institutions worldwide. The EGCF provides access to various instrumentation and equipment and offers fee-based genomic services including sequence and fragment analysis. The EGCF manager is available to provide services and assist with questions regarding lab instrumentation or protocols.

Affiliations:

• Hawai‘i Institute of Marine Biology
• School of Ocean and Earth Science and Technology (SOEST), University of Hawaii at Manoa Organized Research Units

People:

• Director: Eggers, Amy
  
• PI: Gates, Ruth, Ph.D.
  

Resources:

Instruments

• ABI 3130xl genetic analyzer ( DNA sequencer )
  

  "Applied Biosystems 3130xl Genetic Analyzer is the latest generation of 16-capillary electrophoresis instruments for the medium throughput laboratories." 16-capillary electrophoresis instrument used for sequencing analysis.

• Axon GenePix4100 microarray scanner ( Microarray platform )
  

• Beckman Coulter Allegra 25R centrifuge ( Refrigerated centrifuge )
  

• Bio-Rad VersArray colony picker ( Colony picker )
  

• Genome Analyzer IIx ( Next generation sequencer )
  

  "The Genome AnalyzerIIx offers a powerful combination of 2 x 150 bp read lengths and up to 640 million paired-end reads per flow cell, enabling a broad range of high-throughput sequencing applications. Evidenced by a vast number of peer-reviewed publications in an ever-broadening range of applications, Illumina sequencing technology with the Genome AnalyzerIIx is a proven platform for genomic discovery and validation."

• JOUAN S.A. centrifugal evaporator ( Rotary evaporator )
  

  This instrument dries samples.

• LCQ Deca XP Plus spectrometer ( Ion trap mass spectrometer )
  

  This system is used to separate, identify, and quantify compounds.

• MyCycler thermal cycler ( Thermal cycler )
  

  This core facility has three of these instruments.

• Optima MAX benchtop ultracentrifuge ( Ultracentrifuge )
  

  This is a temperature controlled centrifuge for high speed applications.

• Shimadzu HPLC instrument ( High performance liquid chromatography instrument )
  

• StepOnePlus real-time PCR system ( Real-time PCR machine )
  

  This instrument amplifies and quantifies target DNA. Standard cycling takes under 2 hours while fast cycling takes under 40 seconds.

• Zeiss Observer Z1 microscope ( Fluorescence microscope )
  

  The instrument is located on a Newport air table and surrounded by an XL-LSM incubator chamber.

Services

• DNA sequencing service ( Data analysis service )
  

• Fragment analysis service ( Data analysis service )
  

Web Links:

• https://sites.google.com/site/himbcorelab/