Laboratory for Microbial Oceanography

Summary:

The Laboratory for Microbial Oceanography is a research unit within the School of Ocean and Earth Science and Technology (SOEST) at the University of Hawaii at Manoa.

The Laboratory for Microbial Oceanography conducts basic research on microbial inhabitants of the sea, including bacteria, protozoans and unicellular algae. These studies range in scope from the development of novel techniques to assess in situ microbial biomass, activity and growth, to comprehensive field studies designed to elucidate the mechanisms and rates of microbiological cycling of C, N and P.

Affiliations:

• School of Ocean and Earth Science and Technology (SOEST), University of Hawaii at Manoa Organized Research Units

People:

• PI: Karl, David M., Ph.D.
  

Resources:

Instruments

• ABI 7300 real-time PCR system ( Real-time PCR machine )
  

• Agilent 7890A gas chromotograph ( Gas chromatography equipment )
  

  The lab has two of these instruments. "Adding an exciting new chapter to a 40-year history of GC leadership, Agilent’s 7890A GC gives you everything you need to take your lab to the next level of GC and GC/MS performance, including advanced separation capabilities, powerful new productivity enhancements and real-time self-monitoring instrument intelligence. Faster oven cool-down and backflushing let you get more done in less time, at a lower cost per sample. 5th-generation Electronic Pneumatics Control (EPC) and digital electronics set a new benchmark for pressure setpoint and retention time locking precision (0.001 psi) and help make the 7890A our most dependable GC ever."

• Anatech USA 7050 nitric oxide detector ( Nitric oxide monitor )
  

• Beckman Coulter Allegra X-15R centrifuge ( Refrigerated centrifuge )
  

  "The X-15R spins up to three liters of sample at an industry-leading 5,250 x g, with a robust refrigeration system that cools from ambient to 4C in less than 4 minutes – over 10 minutes faster than other three liter centrifuges. Save even more time with optional ARIES rotor technology that accepts up to 50 grams (effectively one tube!) of rotor imbalance, allowing you to eye-balance your samples with confidence. The X-15R is the ideal choice for all your cellular separation applications."

• Beckman Coulter DU 800 UV/Vis spectrophotometer ( UV/vis spectrophotometer )
  

  The lab has two of these instruments.

• Bio-Rad MyCycler thermal cycler ( Thermal cycler )
  

  The lab has 4 of these instruments.

• Bio-Rad PTC-0200G/ALS thermal cycler with 96-well DNA engine ( Thermal cycler )
  

• Bio-Rad PTC0200 DNA thermal cycler ( Thermal cycler )
  

• BioImaging Solutions HG & XE customized field emission scanning electron microscope ( Field emission scanning electron microscope )
  

• BMC B-605 fume hood ( Chemical fume hood )
  

• Brinkman Instruments titration system ( Reagent titrator )
  

• Cytopeia air compressor ( Compressor )
  

• Cytopeia Influx flow cytometer ( Flow cytometer sorter )
  

• Exeter CE440 elemental analyzer ( Chemistry analyzer )
  

• Fasttrackau FRRF6500 submersible fluorometer ( Fluorometer )
  

• Fisher Scientific Fixed 121C sterilizer ( Autoclave )
  

• Fluid Imaging Flowcam VS 6 flow cytometer ( Flow cytometer )
  

• Gel Doc XR imaging system ( Gel imaging system )
  

• iRobot SG1K seaglider ( Autonomous underwater vehicle )
  

• Labconco Delta 6Ft. sterile transfer hood ( Laminar flow hood )
  

• Liquid scinnlation analyzer ( Scintillation counter )
  

• McLane RAS 3-48-500 remote access water sampler ( Water purification system )
  

• Methohm Titrando 809 reagent titrator ( Reagent titrator )
  

• Mettler XP2U Ultramicro balance ( Balance )
  

• Millipore Elix 10 water purification system ( Water purification system )
  

• Millipore Guava Easycyte flow cytometer ( Flow cytometer sorter )
  

• Nikon Eclipse E600 microscope ( Microscope )
  

• Packard Tricarb 1000TR portable liquid scintillation unit ( Scintillation counter )
  

• Paramount Duet 3Ft. filtered fume hood ( Chemical fume hood )
  

• Parr 6765 calorimeter ( Calorimeter )
  

• Sanyo MLS-3751L autoclave ( Autoclave )
  

  The lab contains two of these instruments.

• Satlantic ISUS V2 nitrate analyzer ( Chemistry analyzer )
  

  The lab has two of these instruments.

• Seaglider ( Autonomous underwater vehicle )
  

  The lab has three of these customized seagliders.

• SEAL Analytical XY-2 autosampler ( Autosampler )
  

• Shimadzu GC-8APF gas chromotograph ( Gas chromatography equipment )
  

• Shimadzu TOC-V total organic compound analyzer ( Chemistry analyzer )
  

• Sorvall benchtop centrifuge ( Tabletop centrifuge )
  

• Spectrophotometer ( Spectrophotometer )
  

• Thermo Fisher Scientific DNA120-115 SpeedVac concentrator ( Vacuum centrifuge concentrator )
  

• TRUE FOOD chromatography refrigerator ( Reflectometer )
  

  72 Cubic Foot Chromatography Refrigerator, 3 Swing Doors

• Turner Biosystems luminometer ( Plate luminometer )
  

• Turner Designs 10-AU fluorometer ( Fluorometer )
  

• Turner Designs 7200 fluorometer ( Fluorometer )
  

• VWR Epifluorescent OMEG microscope ( Fluorescence microscope )
  

Web Links:

• http://hahana.soest.hawaii.edu/