RCMI Imaging Core Facility

Directors: Nichols, Robert, Ph.D.; Pitts, Matthew, Ph.D.

Summary:

This core facility provides access to several microscopic platforms. The core also provides technical assistance, training, and consultation in imaging techniques to investigators throughout the University. At present, all of the equipment is available to investigators for use after certified training. Use of the confocal microscopes has an hourly charge.

Affiliations:

• John A. Burns School of Medicine

People:

• Director: Nichols, Robert, Ph.D.
  
• Director: Pitts, Matthew, Ph.D.
  

Member: Lawrence, James
Role: Ph.D. Graduate Student, Training/technical assistance with Li-Cor imaging system and confocal microscopes


Member: Rueli, Rachel
Role: Core Manager, Research Technician, Training/technical assistance with fluorescent microscopes

Resources:

Instruments

• Fujitsu 30A computer workstation for confocal imaging ( Computer workstation )
  

• Li-Cor Odyssey Infrared imaging system ( Infrared image acquisition device )
  

  Two-channel quantitative fluorescent imaging system.

• Nikon AOTF Cameleon ML-4 confocal microscope ( Laser scanning confocal microscope )
  

  Nikon Cameleon 3-Channel AOTF LSM confocal microscope (excitation at 405, 457, 488, 514 and 552nm fully controlled by an acousto-optic tunable filter (AOTF)), with full set of dichroic, band-pass and long-pass filters to allow simultaneous 3-channel recording.)

• Olympus IX71 inverted microscope ( Inverted microscope )
  

  Olympus IX71 inverted fluorescence microscope with Macrofire camera (filter sets for DAPI, FITC/EGFP/BODIPY/DIO, TRITC/Rhodamine/DIL and Texas Red).

• Olympus IX81 DSU confocal microscope ( Spinning-disk confocal microscope )
  

  Olympus spinning disk (DSU) confocal system on a Olympus IX81 with beam splitter and environmental chamber (UV: 330-385nm excitation, 420nm emission; Blue: 450-480nm excitation, 515nm emission; Green: 510-550nm excitation; 590nm emission; FL-Cube for triple channel – all with intermediate dichroics).

• Zeiss Axio 2 Plus fluorescence microscope ( Fluorescence microscope )
  

  Upright Zeiss Axioskop II Plus with AxioCam MRc camera, ASI motorized stage and stereology package (UV: G365nm excitation, LP420nm emission; Blue: BP450-490nm excitation, BP515-565nm emission; Green: BP546nm excitation, LP590 emission – all with intermediate dichroics).

• Zeiss Axio Imager A1 fluorescence microscope ( Fluorescence microscope )
  

  Upright Zeiss Axioimager station with Zeiss AxioCam HRm camera.

• Zeiss Pascal LSM 5 confocal system ( Laser scanning confocal microscope )
  

  Zeiss LSM 5 Pascal confocal system on a Zeiss Axiovert 200M (excitation at 488 and 514 nm (Argon laser), and 543 and 633nm (Helium-Neon laser). A full range of BP, DM and LP filters allow for 2-channel recording.

Protocols

• Overview of stereology ( Protocol )
  

• Primer on cell imaging ( Protocol )
  

Services

• Training and advice for general imaging techniques ( Training service )
  

Web Links:

• http://rcmi-bridges.jabsom.hawaii.edu/MicroscopyC/RCMI-BRIDGES_MicroscopyC.html