Berry Laboratory

Summary:

Research in the Berry lab is focused on selenoproteins, proteins containing the essential trace element, selenium, in the form of an unusual amino acid, selenocysteine. Most selenoproteins in higher organisms function either as antioxidants or in maintaining cellular redox balance. These functions explain the long-known antioxidant properties of selenium. In all selenoproteins whose functions are known, selenocysteine serves as the catalytic center of an enzyme, and its presence is required for optimal enzyme function.

Affiliations:

• Department of Cell and Molecular Biology, John A. Burns School of Medicine

People:

• PI: Berry, Marla, Ph.D.
  

Resources:

Instruments

• Amsco Scientific Century sterilizer ( Autoclave )
  

• Baker SG400 biological safety cabinet ( Biological safety cabinet )
  

• DNA thermal cycler ( Thermal cycler )
  

Organisms and Viruses

• Selenoprotein Binding Protein 2 lentivirus ( Lentivirus )
  

  shRNA against the SBP2

• sh-hSelenoprotein M ( Lentivirus )
  

• sh-hSelenoprotein M ( Lentivirus )
  

• sh-mSelenoprotein M ( Lentivirus )
  

• sh-mSelenoprotein M ( Lentivirus )
  

• sh-Non Target CONTROL GIPZ ( Lentivirus )
  

• sh-Non Target CONTROL TRIPZ ( Lentivirus )
  

• sh-Nucleolin ( Lentivirus )
  

• sh-Phosholipid hydroperoxide glutathione peroxidase ( Lentivirus )
  

• sh-ribonucleic acid binding protein S1 ( Lentivirus )
  

• sh-SecLyase ( Lentivirus )
  

• sh-secp43 ( Lentivirus )
  

• sh-Selenophosphate Synthetase 1 ( Lentivirus )
  

• sh-Selenophosphate Synthetase 1 ( Lentivirus )
  

• sh-Selenophosphate Synthetase 2 ( Lentivirus )
  

• sh-Selenophosphate Synthetase 2 ( Lentivirus )
  

• sh-Selenoprotein 15 ( Lentivirus )
  

• sh-Selenoprotein H ( Lentivirus )
  

• sh-Selenoprotein H ( Lentivirus )
  

• sh-Selenoprotein I ( Lentivirus )
  

• sh-Selenoprotein K ( Lentivirus )
  

• sh-Selenoprotein K ( Lentivirus )
  

• sh-Selenoprotein N ( Lentivirus )
  

• sh-Selenoprotein P ( Lentivirus )
  

• sh-Selenoprotein P ( Lentivirus )
  

• sh-Selenoprotein S ( Lentivirus )
  

• sh-Selenoprotein W ( Lentivirus )
  

• sh-Selenoprotein W ( Lentivirus )
  

• sh-Soluable Liver Antigen-Liver Pancreas/SecS ( Lentivirus )
  

• sh-Thioredoxin reductase Type I ( Lentivirus )
  

• sh-UPF2 regulator of nonsense transcripts homolog ( Lentivirus )
  

Reagents

• pGIPZ-NTC ( Lentiviral plasmid )
  

• pGIPZ-Nucleolin ( Lentiviral plasmid )
  

• pGIPZ-sh-GPX4 ( Lentiviral plasmid )
  

• pGIPZ-sh-hSelM ( Lentiviral plasmid )
  

• pGIPZ-sh-mSelM ( Lentiviral plasmid )
  

• pGIPZ-sh-Rnsp1 ( Lentiviral plasmid )
  

• pGIPZ-sh-SelH ( Lentiviral plasmid )
  

• pGIPZ-sh-SelK ( Lentiviral plasmid )
  

• pGIPZ-sh-SelP ( Lentiviral plasmid )
  

• pGIPZ-sh-SelW ( Lentiviral plasmid )
  

• pGIPZ-sh-SPS1 ( Lentiviral plasmid )
  

• pGIPZ-sh-SPS1 ( Lentiviral plasmid )
  

• pGIPZ-sh-Thioredoxin reductase Type I ( Lentiviral plasmid )
  

• pSM2C-sh-UPF2 ( Lentiviral plasmid )
  

• pTRIPZ-sh-hSelM ( Lentiviral plasmid )
  

• pTRIPZ-sh-mSelM ( Lentiviral plasmid )
  

• pTRIPZ-sh-NTC ( Lentiviral plasmid )
  

• pTRIPZ-sh-SBP2 ( Lentiviral plasmid )
  

• pTRIPZ-sh-SecLy ( Lentiviral plasmid )
  

• pTRIPZ-sh-secp43 ( Lentiviral plasmid )
  

• pTRIPZ-sh-Sel15 ( Lentiviral plasmid )
  

• pTRIPZ-sh-SelH ( Lentiviral plasmid )
  

• pTRIPZ-sh-SelI ( Lentiviral plasmid )
  

• pTRIPZ-sh-SelK ( Lentiviral plasmid )
  

• pTRIPZ-sh-SelN ( Lentiviral plasmid )
  

• pTRIPZ-sh-SelP ( Lentiviral plasmid )
  

• pTRIPZ-sh-SelS ( Lentiviral plasmid )
  

• pTRIPZ-sh-SelW ( Lentiviral plasmid )
  

• pTRIPZ-sh-SLA-LP/SecS ( Lentiviral plasmid )
  

• pTRIPZ-sh-SPS2 ( Lentiviral plasmid )
  

• pTRIPZ-sh-SPS2 ( Lentiviral plasmid )
  

Web Links:

• http://www.hawaii.edu/cmb/berry_lab/