Biological Electron Microscope Facility

Summary:

"The Biological Electron Microscope Facility (BEMF) at the University of Hawai'i is a multi-user/service facility, administered by the Pacific Biosciences Research Center (PBRC). The mission of the BEMF is to provide biological-biomedical researchers with state-of-the-art instrumentation, training and services for high-resolution scanning electron microscopy, conventional and energy-filtering transmission electron microscopy, optical, fluorescence, and laser scanning confocal microscopy, and image analysis on a recharge basis. The facility receives partial support from the Pacific Biosciences Research Center and the UH Manoa Chancellor's Office."

Includes the Richard Allen Image Collection, a unique set of images taken with electron microscopes (http://www5.pbrc.hawaii.edu/allen/).

People:

• PI: Dunlap, Marilyn
  

Member: Carvalho, Tina
Role: Research Associate

Resources:

Instruments

• Hitachi S-4800 FESEM ( Field emission scanning electron microscope )
  

  Service contract available to PI.

• Hummer 6.2 sputter coater ( Automatic sputter coater )
  

• LEO 912 microscope ( Energy filtering transmission electron microscope )
  

  Comes with an Omega energy filter (Zeiss); service contract available to PI.

• Olympus epifluorescence microscope ( Fluorescence compound microscope )
  

  Comes with DIC optic and digital camera.

• Olympus Fluoview microscope ( Laser scanning confocal microscope )
  

  Includes 6 laser lines.

• Olympus stereo microscope ( Stereo microscope )
  

  Comes with digital camera.

• Oxford INCA EDS system ( Spectrophotometer )
  

  Accessory of the Hitachi S-4800 FESEM.

• Reichert KF80 ultrarapid cryofixation system ( Cryofixation device )
  

• Samdri 795 CPD ( Photometer )
  

• Ultracut E ultramicrotome ( Ultratome )
  

  Lab houses two of these instruments.

• Ultracut RMC ultramicrotome ( Ultratome )
  

• Zeiss PALM Combi system ( Laser capture microdissection microscope )
  

  Comes with optical trapping system.

Services

• Conventional and energy-filtering transmission electron microscopy service ( Access service )
  

  Two-tiered price structure (UH & Non-UH).

• Cryofixation service ( Material processing service )
  

• Fluorescence microscopy service ( Access service )
  

  Two-tiered price structure (UH & Non-UH).

• Laser microdissection and laser tweezing service ( Material processing service )
  

  Two-tiered price structure (UH & Non-UH).

• Laser scanning confocal microscopy service ( Access service )
  

  Two-tiered price structure (UH & Non-UH).

• Scanning electron microscopy service ( Access service )
  

  2 tiered price structure (UH & Non-UH)

• Ultramicrotomy service ( Material processing service )
  

Software

• analySIS ( Software )
  

  Image analysis software used with transmission electron microscopy images.

Web Links:

• http://www5.pbrc.hawaii.edu/bemf/site/