Originally from New Zealand, Dr. Collier received her B.Sc. in Pharmacology from the University of Auckland (1998) and her PhD in Pharmacology from the same institution in 2003. Dr. Collier teaches Pharmacology to undergraduate and graduate students as well as Clinical Pharmacology for medical students and was awarded the University of Hawaii Regent’s Medal for Teaching in 2011. Within Pharmacology, her sub-specialty is drug metabolism and pharmacokinetics, primarily of the phase II (conjugation) enzymes, focused on pregnancy and pediatrics. A winner of the 2011 SimCYP award for Most Informative Report (“in recognition of scientific research that is leading the world in ADME, IVIVE, pharmacokinetics, modeling and simulation”), Dr. Collier uses a combination of wet laboratory work and in silico modeling to provide greater understanding of developmental pharmacology and improve drug/chemical safety. Dr. Collier’s research laboratory has been continuously funded by the National Institutes of Health, and private foundations since 2007. Along with her collaborators, she also performs research and publishes regularly in the fields of human and environmental toxicology and in endocrinology.
Lab contains three of these instruments.
Protocol used to study SULT activity.
Protocol used to study enzyme activity.
Protocol used to study UGT1A1 activity.
Protocol used to study GST enzymes.
"The MTT assay is a colorimetric assays for measuring the activity of enzymes that reduce MTT to formazan dyes, giving a purple color. A main application allows to assess the viability (cell counting) and the proliferation of cells (cell culture assays). It can also be used to determine cytotoxicity of potential medicinal agents and toxic materials, since those agents would stimulate or inhibit cell viability and growth."
Protocol used to study oxidative stress.
For protein separation.