Nichols Lab

Summary:

Research focuses on the regulation of the presynaptic nerve terminal:

- Beta amyloid (Aβ) regulation of presynaptic calcium
- Lipid rafts in Aβ regulation
- Nicotine regulation via presynaptic nicotinic receptors
- Calcium homeostasis

Affiliations:

• Department of Cell and Molecular Biology, John A. Burns School of Medicine

People:

• PI: Nichols, Robert, Ph.D.
  

Resources:

Instruments

• ABI DNA sequencer ( DNA sequencer )
  

• Eppendorf AG 5341 thermal cycler ( Thermal cycler )
  

• Eppendorf AG 5702RH centrifuge ( Centrifuge )
  

• Nikon microscope ( Confocal microscope )
  

• Perkin Elmer luminescence spectrometer ( Spectrophotometer )
  

• Wescor Vapro 5520XR osmometer ( Osmometer )
  

• Zeiss Axio Imager A1 upright microscope ( Upright microscope )
  

  Includes auto imaging system.

Protocols

• Calcium imaging of pre-synaptic stucture protocol ( Protocol )
  

Reagents

• HEK-293 ( Immortalized embryonic cell line )
  

• NG 108-15 ( Tumor-derived cell line )
  

  Mouse neuroblastoma cell line.

Software

• PyMOL ( Software )
  

  "PyMOL is a user-sponsored molecular visualization system on an open-source foundation."

Web Links:

• http://www2.hawaii.edu/~robert7/Nichols_Lab/Home.html