SOEST Flow Cytometry Facility

Director: Selph, Karen

Summary:

The Flow Cytometry Facility, operated out of the School for Ocean, Earth Sciences and Technology at the University of Hawaii, provides state of the art instrumentation for analyzing and sorting particles. Flow cytometers detect particles based on their size (scatter) and fluorescence (auto or imparted by a dye). These particles can be enumerated or simply characterized as to their scatter and fluorescence characteristics. Multiple populations can be separated visually and if desired, physically, based on these properties. Flow cytometers work ideally with cells in the 0.2 - 200 µm diameter size range and cell concentrations as low as 1000 cells/ml.

Affiliations:

• School of Ocean and Earth Science and Technology (SOEST), University of Hawaii at Manoa Organized Research Units

People:

• Director: Selph, Karen
  

Resources:

Instruments

• Beckman Coulter Altra research-grade flow cytometer ( Flow cytometer sorter )
  

  5 lasers: 2 UV visible wavelengths laser (including 310nm, 488nm, 514nm), 1 argon ion laser (488 nm), 1 helium-neon laser (543.4 nm), 1 helium-neon laser (633 nm); large particle sorting option.

• Beckman Coulter bench-top analyzer ( Flow cytometer analyzer )
  

• Beckman Coulter Multisizer III cell counter ( Automatic cytometer )
  

• Olympus epifluorescence microscope ( Fluorescence microscope )
  

Services

• Cell cycle analysis service ( Material analysis service )
  

  Service-specific document available on demand.

• Cell cycle analysis with live or dead cells ( Material analysis service )
  

• Fluorescent cell (particle) analysis ( Material analysis service )
  

• Fluorescent cell (particle) sorting service ( Material processing service )
  

• Microplankton analysis service ( Material analysis service )
  

• Quantitative picoplankton analysis ( Material analysis service )
  

  Includes heterotrophic bacteria.

• Traditional blood cell analysis service ( Material analysis service )
  

Software

• Expo32 MultiComp ( Software )
  

• FlowJo ( Software )
  

  Used with the research-grade flow cytometer.
  
  Flow cytometry data and statistical analysis software.

Web Links:

• http://www.soest.hawaii.edu/sfcf/