RCMI Greenwood Molecular Biology Facility

Summary:

The Greenwood Molecular Biology Facility is a primary research infrastructure operated by the Biotechnology Program of the Pacific Biosciences Research Center. The Facility is vital to the educational and research mission of the University of Hawaii by serving the biomedical and molecular biology research and educational programs of the University and its associated institutions. The activities of the the Facility are structured to address both the present and the future needs of researchers for services in genomics, proteomics and bioinformatics.

Affiliations:

• Pacific Biosciences Research Center, University of Hawaii at Manoa

People:

• PI: Mocz, Gabor
  

Resources:

Instruments

• ABI 3130xl genetic analyzer ( DNA sequencer )
  

  "Applied Biosystems 3130xl Genetic Analyzer is the latest generation of 16-capillary electrophoresis instruments for the medium throughput laboratories." Maintenance contracts no longer available due to budget cuts.

• ABI GeneAmp PCR System 9700 ( PCR instrument )
  

  Maintenance contracts no longer available due to budget cuts.
  
  "GeneAmp® PCR System 9700 is specifically designed for the amplification of nucleic acids. The GeneAmp® PCR System 9700 consists of a base module and one of many interchangeable sample block modules. The 96-well GeneAmp® PCR System 9700 is designed for use with 0.2 ml reaction tubes or 96-well reaction plates for all of your routine PCR applications."

• ABI PRISM 377XL DNA sequencer ( DNA sequencer )
  

  Lab houses two of these instruments. Maintenance contracts no longer available due to budget cuts.

• ABI Procise 494 sequencing system ( Protein sequencer )
  

  Maintenance contracts no longer available due to budget cuts. "The Procise® Protein Sequencing System is the gold standard for chemical protein sequencing and protein identification. It delivers picomole sensitivity with a simple load-and-go set-up. Designed for unattended operation overnight or over-the-weekend."

• Affymetrix GeneChip Fluidics Station 450 ( Fluidics station )
  

  "The GeneChip® Fluidics Station 450 is used for the wash and stain operation of Affymetrix GeneChip® arrays. The instrument includes design advancements to provide improved ease of use and true walk-away freedom dramatically improving efficiency in your genetic analysis." Maintenance contracts no longer available due to budget cuts.

• Agilent 2100 Bioanalyzer ( Lab on a chip )
  

  Maintenance contracts no longer available due to budget cuts. "The Agilent 2100 Bioanalyzer is a microfluidics-based platform for sizing, quantification and quality control of DNA, RNA, proteins and cells on a single platform. Results are delivered within 30-40 minutes in automated, high quality digital data."

• Beckman Coulter DU 800 UV/Vis spectrophotometer ( UV/vis spectrophotometer )
  

  Maintenance contracts no longer available due to budget cuts.

• Eppendorf AG Mastercycler Gradient ( PCR instrument )
  

  Maintenance contracts no longer available due to budget cuts.

• Eppendorf AG MiniSpin desktop centrifuge ( Centrifuge )
  

  Maintenance contracts no longer available due to budget cuts.

• Eppendorf AG VacuFuge concentrator ( Vacuum centrifuge concentrator )
  

  Maintenance contracts no longer available due to budget cuts.

• GeneChip Scanner 3000 7G ( Array scanner )
  

  Maintenance contracts no longer available due to budget cuts.
  
  "The GeneChip® Scanner 3000 7G is an extension of the GeneChip® Scanner 3000 (GCS 3000) series that allows you to scan next-generation higher-density arrays, including SNP arrays with up to 900,000 SNPs, tiling arrays for transcription and all-exon arrays for whole-genome analysis.
  
  The GCS 3000 7G combines space-saving and other design improvements with speed, superior performance and lower scanner-to-scanner variation to dramatically improve throughput and efficiency in your genetic analysis. The GCS 3000 7G also provides enhanced auto-focus and more consistent scanner-to-scanner biological performance to improve data integrity and data sharing between researchers."

• iCycler PCR system ( Real-time PCR machine )
  

  Maintenance contracts no longer available due to budget cuts.

• LightTyper system ( Real-time PCR machine )
  

  Maintenance contracts no longer available due to budget cuts.

• NanoDrop spectrophotometer ( Spectrophotometer )
  

  Lab contains two of these instruments. Maintenance contracts no longer available due to budget cuts.

• Stratagene UV StrataLinker ( DNA synthesizer )
  

  Maintenance contracts no longer available due to budget cuts.

• Thermo microcentrifuge ( Centrifuge )
  

  Maintenance contracts no longer available due to budget cuts.

• Transgenomic, Inc. 3500HT wave navigator ( Denaturing high-performance liquid chromatography instrument )
  

  Maintenance contracts no longer available due to budget cuts.

Protocols

• DNA fragment sizing and genotyping protocol ( Protocol )
  

• DNA sequencing protocol ( Protocol )
  

• Oligonucleotide synthesis protocol ( Protocol )
  

• Protein sequencing protocol ( Protocol )
  

• Real-Time PCR analysis protocol ( Protocol )
  

Services

• DNA fragment sizing and genotyping service ( Material analysis service )
  

  No fee for people outside Hawaii.

• DNA microarray analysis service ( Material analysis service )
  

  No fee for people outside Hawaii.

• DNA sequencing service ( Material analysis service )
  

  No fee for people outside Hawaii.

• Molecular modeling service ( Material analysis service )
  

• Protein sequencing service ( Material analysis service )
  

  No fee for people outside Hawaii.

• Real-time PCR analysis ( Material analysis service )
  

  No fee for people outside Hawaii.

Software

• Vector NTI software ( Software )
  

  Comprehensive, fully integrated software package for sequence analysis and data management. The lab has three copies of this software.

Web Links:

• http://www5.pbrc.hawaii.edu/gmbf/