RCMI Pathogen Reference & Reagent Core

Summary:

Our core provides a standardized library of pathogens and reagent materials.

Affiliations:

• John A. Burns School of Medicine

People:

• PI: Nerurkar, Vivek, Ph.D.
  

Member: Cropp, Bruce C., M.S.
Role: Associate Specialist, Head, Pathogen Reference and Reagent Core Facility

Resources:

Instruments

• Bio-Rad Gemini XS reader ( Spectrophotometer )
  

• Biotek microplate reader ( Microplate reader )
  

• Biotek microplate washer ( Microplate washer )
  

• Eppendorf AG 5810R centrifuge ( Refrigerated centrifuge )
  

• Micromass Quattro II ELISA reader ( Spectrophotometer )
  

  Instrument uses the enzyme-linked immunosorbent assay (ELISA).

• MyiQ Single-Color PCR machine ( PCR instrument )
  

• Olympus fluorescence microscope ( Fluorescence microscope )
  

  Camera, computer, and digital storage are connected to the instrument.

• SpectraMax protein reader ( Spectrophotometer )
  

  "The patented multichannel optical design of the SpectraMax® 340PC384 Absorbance Microplate Reader mimics a dual beam spectrophotometer, measures each sample in the plate directly, and eliminates measurement error due to variations in light output between optical fibers on other instruments for more reliable data reporting. The eight-channel system delivers both high precision and speed when reading 96- and 384-well microplates for endpoint measurements, kinetic assays, and spectral scans."

• Sterilizer ( Autoclave )
  

• Thermo Fisher Scientific CO2 water jacketed incubator ( CO2 incubator )
  

Protocols

• Identification of viruses protocol ( Protocol )
  

• Seed virus protocol ( Protocol )
  

• Storage for dengue viruses protocol ( Protocol )
  

Services

• Dengue virus storage service ( Storage service )
  

• Reference antigens and antibodies access service ( Access service )
  

• Viable seed virus service ( Material analysis service )
  

Web Links:

• http://apitmid.hawaii.edu/TCF.htm