This a relatively new lab; funding NIH/NCCAM to research the mechanisms by which selenium influences T Helper Cells during Immune Responses. The major goals of this project are to characterize mechanisms by which Se affects CD4+ T cell activation and identify specific selenoproteins that mediate the effects of Se on CD4+ T cells.
"The X-15R spins up to three liters of sample at an industry-leading 5,250 x g, with a robust refrigeration system that cools from ambient to 4C in less than 4 minutes – over 10 minutes faster than other three liter centrifuges. Save even more time with optional ARIES rotor technology that accepts up to 50 grams (effectively one tube!) of rotor imbalance, allowing you to eye-balance your samples with confidence. The X-15R is the ideal choice for all your cellular separation applications."
"The gentleMACS™ Dissociator is a benchtop instrument for the automated dissociation of tissues. Two types of unique gentleMACS Tubes used with the instrument enable the time-saving and easy dissociation or homogenization of tissues in a closed system."
Measurements: eosinophil count, cytokine secretion in lungs, cellular infiltration, and airway resistance.
Using peptides emulsified in adjuvant; ex-vivo spleen cell restimulation.
From mouse femur and tibiae bone marrow cells and culture to generate macrophage.
Inject either LPS or poly (lic) as a pro-inflammatory reagent; measure cellular infiltration and inflammation cytokine production.
Adjuvants involved: Freund's adjuvant, alum, LANAC (lipid-based adjuvant).
Protocol used for cytokine measurement.
Used to study for cell purification to study inflammation and immune response.
Protocol used for lung physiology measurements.
From mouse femur and tibiae bone marrow cells and culture to generate macrophage.
Tet inducible shRNA expression system.
Depending on which DsRed, some contain sv40.