eagle-i University of Hawai'i ManoaUniversity of Hawai'i Manoa
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COBRE Molecular & Cellular Immunology Core (FACS)


The of the goal of the Immunology Core of MCI, which functions as a fee-for-service facility, is to provide the Hawaii community with a service-oriented facility to meet the needs in the FACS research areas. We are equipped with BD FACSAria, BD FACSCalibur, GuavaEAsyCytePlus, Luminex 200, Beckman-Coulter ViCell, CTL-ImmunoSpot® S5 Core Analyzer, Bio-Rad and BioTek ELx808 ELISA readers and washers, Faxitron compact 43855D x-ray irradiation system, and Faxitron x-ray CP160 for in vivo animal work.





  • BD FACSAria flow cytometer ( Flow cytometer )

    "The BD FACSAria flow cytometer is the first fixed-alignment benchtop high- speed cell sorter. The instrument can be operated at varied pressures and can acquire up to 70,000 events per second. With its fixed-optics design and digital electronics, the BD FACSAria flow cytometer enables multicolor analysis of 7 fluorescent markers and two scatter parameters at a time."

  • BD FACSCalibur flow cytometer ( Flow cytometer )

    "The BD FACSCalibur™ platform offers a unique modular approach to flow cytometry and allows users to perform both cell analysis and cell sorting in an innovative single benchtop system."

  • Beckman Coulter Vi-CELL ( Automatic cytometer )

    "The Vi-CELL Series Cell Viability Analyzers provide an automatic and cost effective means to perform the trypan blue dye exclusion method."

  • C.T.L. ImmunoSpot S5 Core ELISPOT counter ( ELISPOT microplate reader )

    "The S5 Core is CTL’s dedicated model that has been fine tailored for ELISPOT work in the 96 well format. Its features high end optics and cameras – permitting to counting objects as small as >25 µm in size which can be critical for certain ELISPOT assays, including TNF and perforin measurements. The S5 Core ideally lends itself for GLP and high-throughput upgrades. The S5 Core instrument is ideally suited for users who do one particular type of assay while having the highest expectations for image and analysis quality."

  • Faxitron Compact 43855D X-ray irradiation system ( Irradiation system )

  • Guava EasyCyte Plus ( Flow cytometer )

    "Automated multicolor detection and absolute counts for fast and easy identification of cell phenotype and function."

  • Luminex 200 analyzer ( Flow cytometer analyzer )

    "The Luminex 200 System is a flexible analyzer based on the principles of flow cytometry. The system enables you to multiplex (simultaneously measure) up to 100 analytes in a single microplate well, using very small sample volumes. The system delivers fast and cost-effective bioassay results on many assay formats including nucleic acid assays, receptor-ligand assays, immunoassays and enzymatic assays."


  • Apoptosis analysis service ( Material analysis service )

    "FACS is used to study DNA cell content. Propidium iodide (PI) and Hoechst dyes bind to DNA and become fluorescent. PI cannot enter live cells and is included in immunofluorescent staining protocols to identify dead cells. Some Hoechst dyes can enter live cells. DNA staining can be used to study the cell division cycle. Relative DNA content shows the proportion of cells in G1, G2 and S phases. Apoptotic cells show characteristic smear on DNA staining. DNA staining examples:
    * Studying changes in cell cycle in mutant cell
    * Measuring apoptosis in cells after irradiation"

  • Calcium flux analysis service ( Material analysis service )

    "FACS can be used to investigate cell biology. Calcium flux can be measured using Indo-1 markers. This can be combined with immunofluorescent stain. For example, identify T cell subpopulations by immunofluorescence and measure calcium flux in response to an activating signal. Rhodamine-123 stains mitochondrial membranes is used to measure cellular activation. Rhodamine-123 is rapidly pumped out of some cells (for example hematopoietic stem cells)."

  • Cell cycle analysis with live or dead cells ( Material analysis service )

  • Cell population isolation service ( Material processing service )

  • Clinical trials service ( Support service )

  • Drug discovery service ( Analysis service )

  • Fluorescent protein expression service ( Material production service )

  • FRET service ( Material analysis service )

  • High-throughput screening service ( Material analysis service )

  • Hybrid plant development service ( Material processing service )

  • Vaccine validation service ( Material analysis service )

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Last updated: 2011-05-04T08:58:58.587-05:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016