COBRE Transgenic Core

Summary:

"The Transgenic and Embryonic Stem Cell Gene Targeting Core is a state-of-the-art facility with the expertise in the production of genetically altered subjects. Transgenic subjects carrying new or novel genes are created by microinjection of DNA into the pronuclei of fertilized eggs. Knock-out lacking specific genes of interest are created by homologous recombination in embryonic stem cells followed by injection into blastocysts to create chimeric subjects. Highly experienced personnel produce transgenic and knock-out subjects for UH investigators at very reasonable cost and with very short lead times." (http://www.ibr.hawaii.edu/tc.html)

People:

• PI: Ward, W. Steve, Ph.D.
  

Resources:

Instruments

• Narishige MF-900 microforge ( Micropipette )
  

  Lab contains two of these instruments.

• Olympus inverted flurorescent microscope ( Inverted microscope )
  

• Olympus inverted IX71 IVF microscope ( Inverted microscope )
  

• Olympus SZX16 stereo microscope ( Stereo microscope )
  

• Sutter P-97 micropipette puller ( Micropipette puller )
  

Services

• Embryo culture service ( Material processing service )
  

• Embryo freezing service ( Material maintenance service )
  

• Embryo transfer service ( Material production service )
  

• In vitro fertilization (IVF) service ( Material processing service )
  

• Intracytoplasmic sperm injection (ICSI) service ( Material production service )
  

• Transgenic mouse generation ( Material production service )
  

  Mouse oocyte injection, embryo transplantation.

Web Links:

• http://www.ibr.hawaii.edu/tc.html