Fluorescent Imaging and Analysis

eagle-i ID

Resource Type

Properties

1. Fee for service

   Yes

2. Resource Description

   The Zeiss LSM 710 Confocal Laser Scanning Microscope system has a multitude of functions for fluorescent imaging and analysis. Some of the features of this system include the following: - Colocalization of fluorescent components - Fluorescence intensity measurements for any region of interest - Z-Sectioning of specimens for orthogonal & 3D analysis - Time lapse imaging of dynamic processes in living specimens - Fluorescence Resonance Energy Transfer (FRET) - Fluorescence Recovery After Photobleaching (FRAP) - Spectral imaging for the separation of overlapping emission spectra of specimens labeled with more than one fluorescent dye or expressing more than one innate fluorescent protein. The purchase of the Zeiss LSM 710 confocal microscope for HIMB was made possible by a generous donation from Pam Omidyar (financing provided through the School of Ocean and Earth Science and Technology - SOEST).

3. Service Fee URL

   http://www.hawaii.edu/himb/facilities/confocal.html

4. Contact

   Gates, Ruth, Ph.D.

5. Contact

   Farrar, Chris, Ph.D.

6. Related Resource

   Zeiss LSM 710 Confocal Microscope

7. Service Provided by

   Hawai‘i Institute of Marine Biology

8. Related Technique

   Fluorescence recovery after photobleaching

9. Related Technique

   Fluorescence resonance energy transfer