Sequencing Facility

eagle-i ID

Resource Type

Properties

1. Resource Description

   The ASGPB genome infrastructure currently consists of several state-of-the-art capillary-based DNA sequencers and accessory equipment to deal with large sample volumes. The facility can process up to 9000 samples per day with a projected sequence output of 6.75 Million bases. Several prokaryotic and one eukaryotic genome project are currently in progress. The facility offers DNA sequencing services for a wide range of templates including plasmid DNA, PCR fragments, cosmid DNA, and Bacterial Artificial Chromosomes (BACs). Sequencing reactions are performed using Applied Biosystems BigDye terminator chemistry and are run on ABI 3730XL capillary-based DNA sequencers.

2. Contact

   Hou, Shaobin, Ph.D.

3. PI

   Alam, Maqsudul, Ph.D

4. Affiliation

   Center for Advanced Studies of Genomics, Proteomics, & Bioinformatics

5. Website(s)

   http://asgpb.mhpcc.hawaii.edu/sequence/

6. Director

   Alam, Maqsudul, Ph.D