eagle-i University of Hawaii at ManoaUniversity of Hawaii at Manoa
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Calcium flux analysis service

eagle-i ID

http://hawaii.eagle-i.net/i/0000012a-2510-d678-c99c-aef680000004

Resource Type

  1. Material analysis service

Properties

  1. Resource Description
    "FACS can be used to investigate cell biology. Calcium flux can be measured using Indo-1 markers. This can be combined with immunofluorescent stain. For example, identify T cell subpopulations by immunofluorescence and measure calcium flux in response to an activating signal. Rhodamine-123 stains mitochondrial membranes is used to measure cellular activation. Rhodamine-123 is rapidly pumped out of some cells (for example hematopoietic stem cells)."
  2. Contact
    Gurary, Alexandra
  3. Access Restriction(s)
    Researchers must be trained on site or demonstrate core competencies before accessing instrumentation.
  4. Access Restriction(s)
    Restrictions mandated by state or federal law.
  5. Service Provided by
    COBRE Molecular & Cellular Immunology Core (FACS)
  6. Website(s)
    http://cobre.manoa.hawaii.edu/pceidr/mci/flow/guide.html
  7. Related Technique
    Fluorescence activated cell sorting (FACS)
 
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Provenance Metadata About This Resource Record

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016