Calcium flux analysis service

eagle-i ID

Resource Type

Properties

1. Resource Description

   "FACS can be used to investigate cell biology. Calcium flux can be measured using Indo-1 markers. This can be combined with immunofluorescent stain. For example, identify T cell subpopulations by immunofluorescence and measure calcium flux in response to an activating signal. Rhodamine-123 stains mitochondrial membranes is used to measure cellular activation. Rhodamine-123 is rapidly pumped out of some cells (for example hematopoietic stem cells)."

2. Contact

   Gurary, Alexandra

3. Access Restriction(s)

   Researchers must be trained on site or demonstrate core competencies before accessing instrumentation.

4. Access Restriction(s)

   Restrictions mandated by state or federal law.

5. Service Provided by

   COBRE Molecular & Cellular Immunology Core (FACS)

6. Website(s)

   http://cobre.manoa.hawaii.edu/pceidr/mci/flow/guide.html

7. Related Technique

   Fluorescence activated cell sorting (FACS)