Apoptosis analysis service

eagle-i ID

Resource Type

Properties

1. Resource Description

   "FACS is used to study DNA cell content. Propidium iodide (PI) and Hoechst dyes bind to DNA and become fluorescent. PI cannot enter live cells and is included in immunofluorescent staining protocols to identify dead cells. Some Hoechst dyes can enter live cells. DNA staining can be used to study the cell division cycle. Relative DNA content shows the proportion of cells in G1, G2 and S phases. Apoptotic cells show characteristic smear on DNA staining. DNA staining examples: * Studying changes in cell cycle in mutant cell * Measuring apoptosis in cells after irradiation"

2. Contact

   Gurary, Alexandra

3. Access Restriction(s)

   Researchers must be trained on site or demonstrate core competencies before accessing instrumentation.

4. Access Restriction(s)

   Restrictions mandated by state or federal law.

5. Service Provided by

   COBRE Molecular & Cellular Immunology Core (FACS)

6. Website(s)

   http://cobre.manoa.hawaii.edu/pceidr/mci/flow/guide.html

7. Related Technique

   Fluorescence activated cell sorting (FACS)