eagle-i University of Hawaii at ManoaUniversity of Hawaii at Manoa
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Apoptosis analysis service

eagle-i ID


Resource Type

  1. Material analysis service


  1. Resource Description
    "FACS is used to study DNA cell content. Propidium iodide (PI) and Hoechst dyes bind to DNA and become fluorescent. PI cannot enter live cells and is included in immunofluorescent staining protocols to identify dead cells. Some Hoechst dyes can enter live cells. DNA staining can be used to study the cell division cycle. Relative DNA content shows the proportion of cells in G1, G2 and S phases. Apoptotic cells show characteristic smear on DNA staining. DNA staining examples: * Studying changes in cell cycle in mutant cell * Measuring apoptosis in cells after irradiation"
  2. Contact
    Gurary, Alexandra
  3. Access Restriction(s)
    Researchers must be trained on site or demonstrate core competencies before accessing instrumentation.
  4. Access Restriction(s)
    Restrictions mandated by state or federal law.
  5. Service Provided by
    COBRE Molecular & Cellular Immunology Core (FACS)
  6. Website(s)
  7. Related Technique
    Fluorescence activated cell sorting (FACS)
Provenance Metadata About This Resource Record
Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016